| RT-PCR技术检测绵羊卵巢内Ghrelin的表达 |
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| 引用本文： | 斯琴高娃,杜晨光.RT-PCR技术检测绵羊卵巢内Ghrelin的表达[J].中国畜牧兽医,2009,36(6):79-81. |
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| 作者姓名： | 斯琴高娃 杜晨光 |
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| 作者单位： | (内蒙古农业大学职业技术学院， 包头 014109) |
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| 基金项目： | 内蒙古农业大学职业技术学应用发展基金和内蒙古自然科学基金 |
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| 摘 要： | 为了明确Ghrelin在绵羊卵巢有腔卵泡上是否存在表达，本研究利用实时定量RT-PCR技术检测了绵羊卵巢有腔卵泡内的卵母细胞、卵丘细胞和壁层颗粒细胞的Ghrelin蛋白的表达量情况。结果揭示绵羊卵巢有腔卵泡内各类型细胞Ghrelin mRNA的相对表达量大致相同。绵羊卵巢有腔卵泡内各类型细胞Ghrelin蛋白的表达及Ghrelin mRNA的表达模式，尤其是卵母细胞中的表达，揭示这一新型分子在绵羊卵巢上具有潜在的调控作用。
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| 关 键 词： | Ghrelin 绵羊有腔卵泡 实时定量RT-PCR |
RT-PCR Detecting Expression of Ghrelin in the Antral Follicles of Sheep Ovary |
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| Siqingaowa,DU Chen-guang.RT-PCR Detecting Expression of Ghrelin in the Antral Follicles of Sheep Ovary[J].China Animal Husbandry & Veterinary Medicine,2009,36(6):79-81. |
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| Authors: | Siqingaowa DU Chen-guang |
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| Institution: | (Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014109， China) |
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| Abstract: | The possibility of Ghrelin expressed in antral follicles of sheep ovary was investigated in this study,Ghrelin protein was detected in cumulus cells(CCs) and granulosa cells(GCs).Relative real-time reverse-transciptase(RT)-PCR experiments confirmed that the Ghrelin mRNA levels did not show significantly difference.The persistent presence of the Ghrelin signaling system within antral follicles,including oocytes,CCs and GCs,revealed the possibility of a potential regulatory role of this novel molecule during ... |
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| Keywords: | Ghrelin |
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