| 1株产碱性木聚糖酶的芽胞杆菌的分离鉴定及其相关研究 |
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| 引用本文： | 曹要玲,白晓婷,刘辉,李旺,杨明明,龚月生.1株产碱性木聚糖酶的芽胞杆菌的分离鉴定及其相关研究[J].畜牧与兽医,2006,38(9):11-14. |
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| 作者姓名： | 曹要玲 白晓婷 刘辉 李旺 杨明明 龚月生 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西,杨凌,712100;武汉阳光广济医药开发有限公司,湖北,武汉,430062
2. 西北农林科技大学动物科技学院,陕西,杨凌,712100 |
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| 摘 要： | 分离到1株产碱性木聚糖酶的革兰氏阳性菌株,通过菌落形态及16srDNA序列同源性分析,确定该菌株为短小芽孢杆菌。生长条件和产酶条件研究结果显示,最适温度和pH分别为50℃和pH8·0。培养基优化试验显示在有机和无机混合氮源条件下(NH4NO30·57%;牛肉膏1%;蛋白胨0·5%;酵母提取物0·5%;木聚糖0·5%)木聚糖酶产量达到最高(180U/mL)。酶学试验表明最适反应条件为50℃,pH8~9;在pH9的条件下,孵育120min时仍具有75%的活力,表明该酶具有较强的碱耐受性。
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| 关 键 词： | 碱性芽孢杆菌 木聚糖酶 16srDNA |
| 文章编号： | 0529-5130（2006）09-0011-04 |
| 收稿时间： | 05 19 2006 12:00AM |
| 修稿时间： | 2006-05-19 |
Isolation and identification of an alkali-tolerate Bacillus sp. Strain producing xylanase |
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| CAO Yao-ling,BAI Xiao-ting,LIU Hui,LI Wang,YANG Ming-ming,GONG Yue-sheng.Isolation and identification of an alkali-tolerate Bacillus sp. Strain producing xylanase[J].Animal Husbandry & Veterinary Medicine,2006,38(9):11-14. |
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| Authors: | CAO Yao-ling BAI Xiao-ting LIU Hui LI Wang YANG Ming-ming GONG Yue-sheng |
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| Abstract: | An alkali-tolerate Bacillus sp. strain was isolated for producing alkali-tolerate xylanase. The strain was identified as one strain of Bacillus pumilus through 16S rDNA sequencing analysis. The xylanase from the strain had a optimal activity at pH 8.0 and 55 ℃. The xylanase exhibited well alkali-tolerate, retaining 75% activitiesafter 120 mintreatedwithinpH 9.0. Themaximumproductionofthealkali-tolerate xylanase from the strain reached 180 U/mL. |
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| Keywords: | alkali-tolerate Bacillus sp Xylanase 16S rDNA |
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