| 氢氧化钠预处理和酶解牛粪纤维素工艺条件优化 |
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| 引用本文： | 徐静静,李静,凌娟,刘茂昌,燕飞来.氢氧化钠预处理和酶解牛粪纤维素工艺条件优化[J].山东农业科学,2009(11):77-79. |
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| 作者姓名： | 徐静静 李静 凌娟 刘茂昌 燕飞来 |
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| 作者单位： | 1. 西南大学资源环境学院,重庆,400716
2. 西南大学资源环境学院,重庆,400716;重庆市农业资源与环境研究重点实验室,重庆,400716 |
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| 基金项目： | 重庆市北碚区科委科技计划项目，重庆市科委自然科学基金计划资助项目，西南大学博士基金 |
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| 摘 要： | 对氢氧化钠预处理牛粪中纤维素的工艺条件进行了优化研究,各因素较优的水平组合为处理温度95℃、时间3 h、碱浓度3%、固液比1∶12.5。氢氧化钠预处理后,纤维素在总体中所占比例有较大提高,而半纤维素和木质素则有所降低。之后经纤维素酶处理,各因素较优的水平组合为温度55℃、酶液浓度10g/L、底物浓度25 g/L、pH值4.4。
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| 关 键 词： | 牛粪 纤维素 碱预处理 酶解 |
Optimization of Technical Conditions for NaOH Pretreatment and Enzyme Hydrolysis of Cellulose in Cow Dung |
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| XU Jing-jing,LI Jing,LING Juan,LIU Mao-chang,YAN Fei-lai.Optimization of Technical Conditions for NaOH Pretreatment and Enzyme Hydrolysis of Cellulose in Cow Dung[J].Shandong Agricultural Sciences,2009(11):77-79. |
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| Authors: | XU Jing-jing LI Jing LING Juan LIU Mao-chang YAN Fei-lai |
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| Institution: | XU Jing - jing, LI Jing , LING Juan, LIU Mao - chang, YAN Fei - lai (1. College of Resources and Environment, Southwest University, Chongqing 400716, China; 2. Chongqing Key Laboratory of Agricultural Resources and Environment, Chongqing 400716, China) |
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| Abstract: | The conditions for degradation of cellulose in cow dung by NaOH pretreatment and enzyme hydrolysis were optimized.The optimum conditions for NaOH pretreatment were the temperature of 100℃,3-hour treatment,the NaOH concentration of 3% and the solid-liquid ratio of 1︰12.5.After the NaOH pretreatment,the cellulose proportion increased greatly,while the proportion of hemicellulose and lignin reduced.Then the optimum conditions for enzyme hydrolysis were the temperature of 55℃,the enzyme concentration of 10 g/L,the substrate concentration of 25 g/L and pH 4.4. |
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| Keywords: | Cow dung Cellulose NaOH pretreatment Enzyme hydrolysis |
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