| 猪细小病毒X株在不同细胞上增殖效果比较 |
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| 引用本文： | 朱绍辉,张述智,单虎,李志中,魏笑笑,董瑞娥,张浩,李超,夏伟,王晓丽,董平.猪细小病毒X株在不同细胞上增殖效果比较[J].东北农业大学学报,2012(6):6-10. |
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| 作者姓名： | 朱绍辉 张述智 单虎 李志中 魏笑笑 董瑞娥 张浩 李超 夏伟 王晓丽 董平 |
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| 作者单位： | 青岛中仁药业有限公司;青岛宝依特生物制药有限公司;青岛农业大学动物科技学院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2009BADB4B00) |
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| 摘 要： | 为选择猪细小病毒X株增殖的细胞系,对猪细小病毒X株在ST、PK-15、CEF、Vero细胞系上增殖情况进行比较,观察病变时间和病变情况,并分别测定其TCID50和血凝价。结果表明,猪细小病毒X株在这4种细胞上都能产生细胞病变,在ST、PK-15、CEF、Vero细胞上最先开始出现病变的时间分别为24、36、72和48 h,测其在以上4种细胞上的TCID50分别为10-6.4/0.1 mL、10-5.8/0.1 mL、10-2.1/0.1 mL和10-3.2/0.1 mL,HA分别为1 210、1 28、1 23、1 25,因此,建议选用ST细胞作为猪细小病毒X株的增殖细胞系。
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| 关 键 词： | PPVX 细胞 增殖 比较 |
Comparison of effects of porcine parvovirus strain X proliferated on different cells |
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| ZHU Shaohui,ZHANG Shuzhi,SHAN Hu,LI Zhizhong,WEI Xiaoxiao,DONG Rui’e,ZHANG Hao,LI Chao,XIA Wei,WANG Xiaoli,DONG Ping.Comparison of effects of porcine parvovirus strain X proliferated on different cells[J].Journal of Northeast Agricultural University,2012(6):6-10. |
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| Authors: | ZHU Shaohui ZHANG Shuzhi SHAN Hu LI Zhizhong WEI Xiaoxiao DONG Rui’e ZHANG Hao LI Chao XIA Wei WANG Xiaoli DONG Ping |
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| Institution: | 1.Qingdao Zhongren Pharmaceutical Co.,Ltd,Qingdao Shandong 266329,China;2.School of Animal Science and Technology,Qingdao Agriculture University,Qingdao Shandong 266109,China;3.Qingdao Boite Biopharmaceutical Co.,Ltd,Shandong Qingdao 266114,China) |
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| Abstract: | To select the most suitable cell line in which the prolifera effects of porcine PK-15、CEF and Vero cell cultures were compared.The time and characteristics of cytopathic parvovirus strain X is the best,the replication effects of porcine parvovirus strain X in ST,effect(CPE) were observed,and the TCID50 and hemagglutination titer were assayed.The results showed that the porcine parvovirus strain X in ST,PK-15,CEF and Vero cells could all produce CPE,CPE began to appear on the first time was 24,36,72,and 48 h after inoculation.The TCID50 in the four kinds of cell cultures were 10-6.4/0.1 mL,10-5.8/0.1 mL,10-2.1/0.1 mL and 10-3.2/ 0.1 mL,and the HA were 1:210,1:28,1:23 and 1:25.Therefore,the final selection was that ST cell line was regarded as the suitable cell line for the replication of porcine parvovirus strain X. |
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| Keywords: | porcine parvovirus(PPV)X cell line proliferation comparison |
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