| 猪伪狂犬病PCR诊断方法的建立 |
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| 引用本文： | 陈钟鸣,张定东.猪伪狂犬病PCR诊断方法的建立[J].动物医学进展,2006,27(2):83-85. |
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| 作者姓名： | 陈钟鸣 张定东 |
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| 作者单位： | 金陵科技学院动物科学系,江苏南京,210038;金陵科技学院动物科学系,江苏南京,210038 |
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| 摘 要： | 根据伪狂犬病病毒(PRV)gB基因的序列,设计并合成了一对引物,以闽A株细胞培养毒为模板,筛选最佳反应条件,建立了检测PRV的PCR方法,应用本方法对分离的病毒进行基因扩增,获得了217bp的特异性DNA片段,证明了对分离病毒检测的特异性和敏感性,为伪狂犬病的快速诊断提供了条件。
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| 关 键 词： | 多聚酶链式反应 伪狂犬病病毒 |
| 文章编号： | 1007-5038（2006）02-0083-03 |
| 收稿时间： | 2005-10-17 |
| 修稿时间： | 2005年10月17 |
The Establishment of PCR Diagnositic Technique of Porcine Pseudorabies |
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| CHEN Zhong-ming,ZHANG Ding-dong.The Establishment of PCR Diagnositic Technique of Porcine Pseudorabies[J].Progress In Veterinary Medicine,2006,27(2):83-85. |
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| Authors: | CHEN Zhong-ming ZHANG Ding-dong |
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| Institution: | Departraent of Animal Science, Jinling Institute of Technology, Nanj ing, Jiangsu, 210038, China |
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| Abstract: | One pair of pri mers that amplifiedthe gBgene of Pseudorabies virus(PRV) was designed and syn-thesized,PCRtechnique detectingthe DNAof PRV was established after selectingthe best reaction condi-tions.This technique was applied to specifically amplify the 217 bp DNAfragment of the PRV.The re-sults showed that the establishment of PRV PCRtechnique provided a more sensitive,specific and reliablemethod for pseudorabies diagnosis. |
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| Keywords: | PCR Pseudorabies virus |
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