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Spectrophotometric methods to determine carbohydrates in soil
Authors:Don A Doutre  George W Hay  Alan Hood  Gary W Vanloon
Institution:

aDepartment of Chemistry, Queen's University, Kingston, Ontario, Canada, K7L 3N6

Abstract:Two common spectrophotometric procedures for determining neutral carbohydrates—the phenol-sulfuric acid and anthrone methods were evaluated for soil analysis. The phenol-sulfuric acid procedure gives results comparable to those obtained by gas-liquid chromatography, and is applicable to a variety of soil types. Prior removal of interfering substances by anion and cation exchange is required. Glucose is recommended as the spectrophotometric reference standard. The anthrone method is not satisfactory under the conditions employed.
Keywords:Corresponding Author  
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