Bioavailability of lysine in selected foods by rat growth assay |
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| Authors: | F E McDonough C E Bodwell A D Hitchins R S Staples |
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| Institution: | (1) Agricultural Research Service, Beltsville Human Nutrition Research Center, Energy & Protein Nutrition Laboratory, U.S. Dept. of Agriculture, 20705 Beltsville, MD, U.S.A. |
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| Abstract: | Lysine bioavailabilities in reference protein and 16 test protein diets were estimated using 10 day rat growth assays. A standard growth curve was obtained by feeding 5 diets containing casein, zein and synthetic amino acids ranging in total lysine concentration from 0.3 to 0.7%. Experimental foods were added to the basal diet at the expense of zein and/or synthetic amino acids to provide 2 specific lysine concentrations, i.e., 0.4 and 0.6%. Availabilities were established by comparing growth responses from the test food diets to the regression line of the standard growth data. Availabilities were over 88% for 13 of 16 products. Utilization was poor in pinto beans (73%), rice-wheat gluten cereal (70%), and skim milk powder heated to 100°C for 12 h (66%). Addition of excess lysine (700 mg/100 g diet) to the pinto bean diet did not improve growth response; thus poor digestibility or some unidentified growth inhibitor is indicated. |
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| Keywords: | protein quality amino acids lysine |
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