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| 肉鸭中黄病毒的分离与鉴定 | |
| 引用本文： | 赖芳芳,陈孝明,张健.肉鸭中黄病毒的分离与鉴定[J].中国畜牧兽医,2013,40(2):179-182. |
| 作者姓名： | 赖芳芳 陈孝明 张健 |
| 作者单位： | 1. 华南农业大学,广东广州,510642 2. 广东现代农业集团研究院,广东广州,510630 3. 广东省农业科学院兽医研究所,广东广州,510640 |
| 基金项目： | 广东省科技厅项目(2011A090400011) |
| 摘 要： | 为探索2011年年底在广东阳江地区某肉鸭场出现的病鸭和死鸭的发病原因,对其中2个发病鸭场的病死鸭进行了解剖,对病变组织的样品应用RT-PCR的方法进行了病原鉴定。经剖检,发现鸭心脏表面形成小结节,伴有少量心包积液;肝脏和胆囊肿大;脾脏有花斑;胰腺和直肠有出血点。利用病料研磨上清和病料接种后的鸡胚尿囊液进行RT-PCR检测,结果显示鸭黄病毒阳性。序列分析显示,该病毒的核苷酸序列与坦布苏病毒Fengxian株的相似性为99%。试验结果表明,该病毒为黄病毒,是引起此次鸭场发病的病原。 |
| 关 键 词： | 鸭黄病毒 RT-PCR 序列分析 |
| 收稿时间： | 2012-08-07 |
Isolation and Identification of Duck Flavivirus |
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| LAI Fang-fang , CHEN Xiao-ming , ZHANG Jian-fei , LUO Jun , CHEN Shan-zhen , WANG Gui-ping , LI Zhong-sheng.Isolation and Identification of Duck Flavivirus[J].China Animal Husbandry & Veterinary Medicine,2013,40(2):179-182. | |
| Authors: | LAI Fang-fang CHEN Xiao-ming ZHANG Jian-fei LUO Jun CHEN Shan-zhen WANG Gui-ping LI Zhong-sheng |
| Institution: | 1. South China Agricultural University, Guangzhou 510642, China;2. Guangdong Institute of Modern Agricultural Group, Guangzhou 510630, China;3. The Veterinary Research Institute of Guangdong Provincial Academy of Agricultural Sciences, Guangzhou 510640, China |
| Abstract: | At the end of 2011, a new duck infectious disease emerged in Guangdong province. In order to identify the etiological agent, the pathological examination and RT-PCR were carried out. By autopsy, we found that cardiac sarcoidosis, accompanied with a few pericardial effusion; liver and gallbladder swelled; spleen was slightly mottled; pancreas and rectum with hemorrhagic spots. The samples from affected ducks and the allantoic fluid of affected chick embryos, were detected positive by duck flavivirus RT-PCR method. Sequence analysis showed that the isolated virus shared 99% nucleotide identity with duck flavivirus Fengxian strain. These results suggested that the isolated virus belonged to duck flavivirus and was the reason for the infectious disease of duck. |
| Keywords: | duck flavivirus RT-PCR sequence analysis |
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