| 羊链球菌CVCC55001、CVCC55002株的制备与鉴定 |
| |
| 引用本文： | 张一帜,任小侠,李 建,王秀丽,刘元杰,李俊平,张 媛.羊链球菌CVCC55001、CVCC55002株的制备与鉴定[J].中国兽药杂志,2021,55(3):11-16. |
| |
| 作者姓名： | 张一帜 任小侠 李 建 王秀丽 刘元杰 李俊平 张 媛 |
| |
| 作者单位： | 中国兽医药品监察所,,,,,,中国兽医药品监察所 |
| |
| 摘 要： | 为研究羊链球菌CVCC55001、CVCC55002株的菌种特性,制备了新的菌种批并对其形态及生化特性、培养特性、血清学特性、真空度、纯粹、剩余水分、毒力及免疫原性等进行鉴定,对其适宜培养基进行了筛选。试验结果表明,该冻干菌种的形态及生化特性、培养特性、血清学特性、真空度、纯粹、剩余水分、毒力及免疫原性均符合《中华人民共和国兽用生物制品规程》二〇〇〇版质量标准的规定。在免疫原性鉴定中,未免疫羊的最小致死剂量:CVCC55001为1.2×104 CFU,CVCC55002为2.6×104 CFU,免疫组能够被有效保护。使用不同培养基对该菌进行培养比对,证实血清为该菌必需成分。本研究可为羊链球菌的制备和鉴定提供参考,也为该菌的稳定培养和疫苗工艺改进提供了研究基础。
|
| 关 键 词： | 羊链球菌 毒力 免疫原性 培养基筛选 |
| 收稿时间： | 2020/10/19 0:00:00 |
| 修稿时间： | 2021/2/4 0:00:00 |
Preparation and Verification of CVCC55001 and CVCC55002 Strains of Streptococcus Ovis |
| |
| ZHANG Yi-zhi,REN Xiao-xia,LI Jian,WANG Xiu-li,LIU Yuan-jie,ZHANG Yuan.Preparation and Verification of CVCC55001 and CVCC55002 Strains of Streptococcus Ovis[J].Chinese Journal of Veterinary Drug,2021,55(3):11-16. |
| |
| Authors: | ZHANG Yi-zhi REN Xiao-xia LI Jian WANG Xiu-li LIU Yuan-jie ZHANG Yuan |
| |
| Institution: | China Institute of Veterinary Drug Control,,,,,, |
| |
| Abstract: | In order to study the characteristics of Streptococcus ovis CVCC55001 and CVCC55002 strains, a batch of CVCC55001 and CVCC55002 strains were prepared, and the morphology and biochemical characteristics, culture characteristics, serological characteristics, vacuum degree, purity, residual moisture, Virulence and immunogenicity of the strains were prepared, and the appropriate medium for the bacteria was screened. The test results showed that all the verification items of the two strains were in compliance with the quality standard of "Regulations for Veterinary Biological Products of the PRC, 2000 version . In the immunogenicity test, the minimum lethal dose of unimmunized sheep is CVCC55001: 1.2×104 CFU; CVCC55002: 2.6×104 CFU, and the immunized group can be effectively protected. Different culture media were compared for the bacteria, and it was confirmed that the serum was essential for the bacteria culture. This study provides a reference for the preparation and verification of Streptococcus ovis, and media comparison provides the research basis for the culture stability of the bacteria and the improvement of vaccine technology. |
| |
| Keywords: | Streptococcus ovis virulence immunogenicity medium selection |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《中国兽药杂志》浏览原始摘要信息 |
| 点击此处可从《中国兽药杂志》下载免费的PDF全文 |
|
