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采后核黄素处理对苹果青霉病及活性氧和苯丙烷代谢的影响
引用本文:蒋超男,李灿婴,李伊涵,寇程程,葛永红.采后核黄素处理对苹果青霉病及活性氧和苯丙烷代谢的影响[J].保鲜与加工,2021,21(3):1-7.
作者姓名:蒋超男  李灿婴  李伊涵  寇程程  葛永红
作者单位:渤海大学食品科学与工程学院,辽宁 锦州 121013;生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁 锦州 121013
基金项目:国家自然科学基金项目(31801595);辽宁省科学技术计划项目(2017205002)
摘    要:以“金冠”苹果果实为材料,采后用不同浓度核黄素溶液浸泡处理,研究其对损伤接种青霉(Penicillium expansum)果实的控制效果及果实苯丙烷代谢途径、活性氧代谢关键酶活性和产物含量的影响。结果表明,低浓度的核黄素处理可显著降低损伤接种P.expansum果实的病斑直径,其中以1.0 mmol/L核黄素处理效果最佳。同时,1.0 mmol/L核黄素处理显著提高了果实过氧化氢酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶、苯丙氨酸解氨酶及过氧化物酶活性,促进了过氧化氢、总酚、类黄酮和木质素的积累。由此表明,采后核黄素处理通过激活苯丙烷途径和活性氧代谢诱导苹果对青霉病的抗性。

关 键 词:苹果  核黄素  活性氧  诱导抗性  扩展青霉

Effects of Riboflavin Treatment on Blue Mould and Reactive Oxygen Species and Phenylpropanoid Metabolisms in Postharvest Apples
JIANG Chao-nan,LI Can-ying,LI Yi-han,KOU Cheng-cheng,GE Yong-hong.Effects of Riboflavin Treatment on Blue Mould and Reactive Oxygen Species and Phenylpropanoid Metabolisms in Postharvest Apples[J].Storage & Process,2021,21(3):1-7.
Authors:JIANG Chao-nan  LI Can-ying  LI Yi-han  KOU Cheng-cheng  GE Yong-hong
Institution:(College of Food Science and Engineering,Bohai University,Jinzhou 121013,China;National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Jinzhou 121013,China)
Abstract:Postharvest apple fruits (cv. Golden delicious) were used as the material and treated with different concentrations of riboflavin solution, then the control effects on fruits with Penicillium expansum inoculation, the changes of key enzymes activities and metabolites content in phenylpropanoid pathway and reactive oxygen species metabolism were further investigated. The results demonstrated that riboflavin treatment at low concentration significantly inhibited lesion diameter in apple fruits inoculated with P.expansum, especially treated with 1.0 mmol/L riboflavin. Moreover, 1.0 mmol/L riboflavin treatment significantly improved the activities of catalase, ascorbate peroxidase, glutathione reductase, phenylanine ammonia lyase and peroxidase, promoted the accumulation of hydrogen peroxide, total phenolic, flavonoid and lignin in apple fruits. These findings suggested that riboflavin treatment on postharvest apple fruits could enhance their disease resistance against blue mould by activating phenylpropanoid pathway and reactive oxygen species metabolism
Keywords:apple  riboflavin  reactive oxygen species  induced resistance  Penicillium expansum
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