| 七类消毒剂对非洲猪瘟病毒荧光定量PCR检测结果的影响 |
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| 引用本文： | 南文龙,巩明霞,陆游,李林,王清华,吴晓东,陈义平.七类消毒剂对非洲猪瘟病毒荧光定量PCR检测结果的影响[J].中国动物检疫,2021,38(3):87-91. |
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| 作者姓名： | 南文龙 巩明霞 陆游 李林 王清华 吴晓东 陈义平 |
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| 作者单位： | 中国动物卫生与流行病学中心;扬州大学兽医学院 |
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| 基金项目： | 国家重点研发计划项目（2018YFC0840401）；湖南创新型省份建设专项（2019NK2181） |
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| 摘 要： | 为评价戊二醛、酚、含碘类等常用消毒剂消毒后对非洲猪瘟病毒荧光定量PCR检测结果的影响,基于畜禽栏舍、运载工具、器具消毒及皮肤黏膜消毒目的,按消毒剂说明书推荐选择不同工作浓度,分别与不同滴度的非洲猪瘟病毒培养物于20℃条件下作用30 min后,采用荧光定量PCR方法检测作用后产物。结果显示,与对应的阳性对照组相比,含氯类(二氯异氰尿酸钠)、过硫酸氢钾类、二氧化氯类消毒剂,消毒后对荧光定量PCR检测结果影响最显著,检测Ct值显著上升或检测不到;戊二醛类、含碘类(主要成分聚维酮碘)消毒剂,核酸降解能力相对较弱,检测Ct值稍有上升;酚类、季铵盐类、含碘类(主要成分碘、磷酸、硫酸)类消毒剂,检测Ct值基本无变化。本研究评价了7类常用消毒剂消毒对非洲猪瘟病毒荧光定量PCR检测结果的影响,可为防控实践中科学、客观评价分析消毒效果提供技术参考。
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| 关 键 词： | 非洲猪瘟 消毒剂 荧光定量PCR 消毒效果 |
The Influence of Seven Categories of Disinfectants on the Test Results of ASFV by the Fluorescent Quantitative PCR |
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| Nan Wenlong,Gong Mingxi,Lu You,Li Lin,Wang Qinghu,Wu Xiaodong,Chen Yiping.The Influence of Seven Categories of Disinfectants on the Test Results of ASFV by the Fluorescent Quantitative PCR[J].China Journal Of Animal Quarantine,2021,38(3):87-91. |
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| Authors: | Nan Wenlong Gong Mingxi Lu You Li Lin Wang Qinghu Wu Xiaodong Chen Yiping |
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| Institution: | (China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China;College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China) |
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| Abstract: | In order to evaluate the influence of common disinfectants including glutaraldehyde,phenol and iodine on the test results of African swine fever virus(ASFV),the disinfectants with different concentrations were used according to the corresponding instructions and based on the purpose of disinfecting pens,vehicles,instruments and skin mucous membrane to react respectively with the cultures of ASFV with different titers at 20℃for 30 min,then the products were tested by fluorescent quantitative PCR.The results showed that,compared to the positive control group,test results of using chlorine(sodium dichloroisocyanurate),potassium bisulfate and chlorine dioxide compound disinfectants were significantly affected,the Ct value significantly increased or failed to be detected;for glutaraldehyde and iodine compound disinfectants(mainly contained povidone iodine),the capability of nucleic acid degradation was relatively lower,and the Ct value slightly increased;for phenol,quaternary ammonium and iodine compound disinfectants(mainly contained iodine,phosphoric acid and sulfuric acid),the Ct value generally remained the same.In conclusion,the influence of seven categories of disinfectants on the test results of ASFV by fluorescent quantitative PCR was evaluated,which could provide some technical references for scientific and objective evaluation and analysis of disinfection effect in practice. |
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| Keywords: | ASF disinfectant fluorescent quantitative PCR disinfection effect |
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