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鸡胚疫苗注射时蛋壳力学模型分析及参数优化
引用本文:王红彬,赵明岩,胡剑虹,王宗炼,宋天月,刘弘业,李运良,陶伟鹏.鸡胚疫苗注射时蛋壳力学模型分析及参数优化[J].农业工程学报,2021,37(4):106-113.
作者姓名:王红彬  赵明岩  胡剑虹  王宗炼  宋天月  刘弘业  李运良  陶伟鹏
作者单位:中国计量大学机电工程学院,杭州 310018
基金项目:国家自然科学基金项目(No.51605462);浙江省自然科学基金(No.LQ16E050001)
摘    要:针对鸡胚疫苗注射时由针头冲击产生裂纹导致接种失败的问题,建立了蛋壳注射时冲击固体力学分析模型。首先,由冲量定理和动量守恒定律推导出注射孔单位长度所受合力与注射初速度、注射体系质量及针头锥形角之间的关系。由ANSYS19.2有限元分析软件初步确定注射初速度的范围为0.5~0.8 m/s,注射体系质量0.5~1.2 kg,针头锥形角为10°和15°。其次,通过图像处理技术实现对注射孔裂纹特征的提取,计算注射孔裂纹有效面积指数和裂纹有效轮廓指数,实现对注射裂纹蛋胚和完好蛋胚的判别。最后采用三因素二水平有交互作用的方差分析法,确定最优注射参数组合:注射初速度为0.6 m/s、针头锥形角为10°、注射体系质量为1.0 kg。采用7~14日龄乌鸡孵化种蛋80枚,根据优化后的参数组合,进行鸡胚注射试验,注射成功率为98.75%。

关 键 词:裂纹  力学模型  参数优化  有限元分析  图像处理  鸡胚  注射
收稿时间:2020/7/25 0:00:00
修稿时间:2021/1/20 0:00:00

Analysis of eggshell mechanical model and parameter optimization for chicken embryo vaccine injection
Wang Hongbin,Zhao Mingyan,Hu Jianhong,Wang Zonglian,Song Tianyue,Liu Hongye,Li Yunliang,Tao Weipeng.Analysis of eggshell mechanical model and parameter optimization for chicken embryo vaccine injection[J].Transactions of the Chinese Society of Agricultural Engineering,2021,37(4):106-113.
Authors:Wang Hongbin  Zhao Mingyan  Hu Jianhong  Wang Zonglian  Song Tianyue  Liu Hongye  Li Yunliang  Tao Weipeng
Institution:China Jiliang University, Hangzhou, 310018, China
Abstract:Abstract: Aiming at to solve the problem of inoculation failure caused by the cracks and the needle impact during the injection of chicken embryo vaccine, a mechanical model of impact during eggshell injection was established and analysied. First, the relationship between the resultant force per unit length of the injection hole and the initial injection velocity, the mass of the injection system and the cone angle of the needle was derived from the impulse theorem and the law of conservation of momentum. In order to verify the correctness of the mechanical model, and to further explore the relationship between eggshell deformation, stress and strain with taper angle, initial injection speed and mass of the injection system during the injection of chicken embryos, ANSYS software was used to simulate the impact of the needle on the eggshell. The simulation results verified the accuracy of the mechanical model, and it was preliminarily determined that the range of the initial injection velocity was 0.5-0.8 m/s, and the range of the injection system mass was 0.5-1.2 kg. The needle cone angles are 10°and 15°. Second, in order to complete the chicken embryo injection test, a chicken embryo injection and micro-crack detection device was established. The device consists of the high-speed camera, injection system, buffer mechanism and crack detection device of the injection hole of chicken embryo. The high-speed camera can measure the initial speed of injection, and the injection system can realize the injection of chicken embryo, even can change the mass of the injection system arbitrarily, and select different specifications the needle can change the cone angle, and the buffer mechanism can effectively avoid the crack of chicken embryo due to vibration. The crack detection device is mainly composed of CCD industrial camera, egg support and cold light source, which can ensure the accurate identification of chicken embryo crack and the smooth progress of injection parameter optimization test. Furthermore, an orthogonal experiment was designed to extract the characteristics of the injection hole cracks with image processing technology, and calculate the injection hole crack effective area index and the crack effective contour index, judgment whether the injection of cracked egg embryos and intact egg embryos. Eggshell was a micro porous structure, and the permeability of the eggshell was inconsistent. The eggshell need to be irradiated with a cold light source during the image processing, and the collected source image has bright spots. Eggshell bright spots cannot be effectively removed, and effective crack features cannot be retained using traditional threshold segmentation algorithm. Therefore, an eggshell bright spot elimination algorithm was used to retain the crack features of the chicken embryo injection hole and remove the bright spots to the maximum extent. Finally, a three-factor two-level interaction analysis of variance method was used to determine the optimal injection parameter combination: the initial injection velocity was 0.6 m/s, the needle cone angle was 10°, and the injection system mass was 1.0 kg. 7-14 day-old chicken embryos were used to test under the optimized parameters, the injection success rate was 98.75%.
Keywords:crack  mechanical model  parameter optimization  ANSYS analysis  image processing  chicken embryo  injection
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