| 固氮芽孢杆菌N3的筛选鉴定及其对二月兰的促生效果 |
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| 引用本文： | 魏志敏,孙斌,方成,代子雯,刘满强,焦加国,胡锋,李辉信,徐莉.固氮芽孢杆菌N3的筛选鉴定及其对二月兰的促生效果[J].土壤,2021,53(1):64-71. |
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| 作者姓名： | 魏志敏 孙斌 方成 代子雯 刘满强 焦加国 胡锋 李辉信 徐莉 |
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| 作者单位： | 南京农业大学,南京农业大学 |
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| 基金项目： | 国家绿肥产业技术体系项目(CARS-22-G-10) |
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| 摘 要： | 从山东泰安农田土壤中筛选获得1株固氮能力强的菌株N3,通过形态观察、生理生化特征以及16S rDNA基因序列分析,确定为巨大芽孢杆菌属(Bacillus megaterium),该菌株固氮酶活性达C2H428.33 nmol/(h·ml).温室条件下进行二月兰盆栽试验,设置不接菌对照(CK)、接种巨大芽孢杆菌N3、接种...
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| 关 键 词： | 植物促生菌 固氮 吲哚乙酸(IAA) 二月兰 |
| 收稿时间： | 2019/9/12 0:00:00 |
| 修稿时间： | 2019/11/9 0:00:00 |
Isolation and Characterization of Bacillus Nitrogen-Fixing Bacteria and Its GrowthPromotion on Orychophragmus violaceus |
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| WEI Zhimin,SUN Bin,FANG Cheng,DAI Ziwen,LIU Manqiang,JIAO Jiaguo,HU Feng,LI Huixin,XU Li.Isolation and Characterization of Bacillus Nitrogen-Fixing Bacteria and Its GrowthPromotion on Orychophragmus violaceus[J].Soils,2021,53(1):64-71. |
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| Authors: | WEI Zhimin SUN Bin FANG Cheng DAI Ziwen LIU Manqiang JIAO Jiaguo HU Feng LI Huixin XU Li |
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| Institution: | Nanjing Agriculture University,Nanjing Agriculture University |
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| Abstract: | A strain N3 with strong nitrogen fixation ability has been selected from the farmland soil in Tai ''an, Shandong province. It was identified as Bacillus megaterium based on the morphological, physiological and biochemical characteristics and 16S rDNA gene sequences analysis. And its nitrogenase activity was up to C2H4 28.33nmol/(h.ml). Four treaments were set to explore the effect inoculation of different plant growth-promoting bacterium on the growth of Orychophragmus violaceus, including single or double inoculation of strain N3 or IAA (indole-3-acetic acid)-producing stain Pantoea agglomerans CDL29, plant without any inoculation set as the control treatment (CK). The pot experiment of Orychophragmus violaceus was conducted under greenhouse conditions. The results showed that all plant growth-promoting bacterium promoted the growth of Orychophragmus violaceus, and the treatment with single inoculation of nitrogen-fixing strain N3 was the best. At the same time, the content of nitrogen, phosphorus in shoots of the Orychophragmus violaceus, and the nitrogen, potassium content in the roots of the Orychophragmus violaceus were significantly increased by the single inoculation of nitrogen-fixing strain N3, which were 33.03%, 55.56% and 29.87% , 51.11% respectively improved compared with the CK treatment. Other treatments also promoted the content of nutrients in Orychophragmus violaceus, but the trends were not statistic significant. The contents of soil organic matter , total nitrogen and available nutrients were significantly increased by single inoculation of nitrogen-fixing strain N3. The contents of soil microbial carbon (MBC) and microbial nitrogen (MBN) were increased by the inoculation of different plant probiotics, single inoculation of nitrogen-fixing strain N3 had the best effect, followed by the single inoculation of IAA-producing stain CDL29 and compound inoculation. In addition, inoculation of nitrogen-fixing strain N3 can significantly increase soil nitrogenase activity, while inoculation of CDL29 had no significantly effect on soil nitrogenase activity. As a conclusion, the single inoculation of nitrogen-fixing strain N3 could significantly promote the growth, nutrient content in Orychophragmus violaceus, and also improve the physicochemical and biological properties of the soil. These experiment results could provide a theoretical and research basis for the application of nitrogen fixation strain N3 in the planting of Orychophragmus violaceus. |
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| Keywords: | Plant growth-promoting bacterium Nitrogen fixation IAA Orychophragmus violaceus |
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