| HPLC-PDA法测定兽用中药液体制剂中非法添加的四种解热镇痛类药物 |
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| 引用本文： | 徐汀,张亦菲,黄家莺,张浩然,田恺,华贤辉,曹莹.HPLC-PDA法测定兽用中药液体制剂中非法添加的四种解热镇痛类药物[J].中国兽药杂志,2021,55(3):30-37. |
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| 作者姓名： | 徐汀 张亦菲 黄家莺 张浩然 田恺 华贤辉 曹莹 |
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| 作者单位： | 上海市兽药饲料检测所,,,,,,上海市兽药饲料检测所 |
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| 摘 要： | 实验建立了兽用中药液体制剂中非法添加对乙酰氨基酚、安乃近、氨基比林、安替比林四种解热镇痛类药物的高效液相色谱-二极管阵列(HPLC-PDA)检测方法。方法采用CAPCELL PAK MGⅡS5 C18(4.6 mm×150 mm,5μm)色谱柱,柱温30℃,流动相为磷酸盐缓冲液和乙腈(84:16),流速1.0 mL/min,进样体积10μL,采集波长范围为190~400 nm,检测波长229 nm。结果显示,对乙酰氨基酚、安乃近、安替比林、氨基比林在浓度分别在1.17~117.28、2.03~202.92、5.58~111.60、2.42~120.80μg/mL范围内线性良好,相关系数R 2分别为0.9986、0.9999、0.9980、0.9993,平均加标回收率(n=5)分别为93.15%~104.25%、93.79%~104.62%、96.96%~99.17%、94.66%~99.83%,RSD分别为0.46%~2.29%、0.30%~3.15%、0.12%~0.94%、0.37%~1.42%(n=5)。该方法便捷,快速,准确,可用于兽用中药液体制剂中非法添加四类解热镇痛类药物的筛查检测。
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| 关 键 词： | 解热镇痛药 中药液体制剂 非法添加 HPCL-PDA |
| 收稿时间： | 2020/8/7 0:00:00 |
| 修稿时间： | 2021/2/4 0:00:00 |
Determination of four antipyretic and analgesic drugs illegally added in liquid preparations of veterinary traditional Chinese medicine by HPLC-PDA |
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| XU Ting,ZHANG Yi-fei,HUANG Jia-yin,ZHANG Hao-ran,TIAN Kai,HUA Xian-hui,CAO Ying.Determination of four antipyretic and analgesic drugs illegally added in liquid preparations of veterinary traditional Chinese medicine by HPLC-PDA[J].Chinese Journal of Veterinary Drug,2021,55(3):30-37. |
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| Authors: | XU Ting ZHANG Yi-fei HUANG Jia-yin ZHANG Hao-ran TIAN Kai HUA Xian-hui CAO Ying |
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| Institution: | Shanghai Institute for Veterinary Drugs and Feeds Control,Shanghai,201103;China,,,,,, |
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| Abstract: | This experiment by a HPLC-PDA detection method was established for determining the illegal addition of four antipyretic and analgesic drugs:paracetamol,paraben,aminopyrine,and antipyrine in liquid preparations of veterinary traditional Chinese medicine.The method used a CAPCELL PAK MGⅡS5 C18(4.6 mm×150 mm,5μm)chromatographic column,the column temperature was 30℃,the mobile phase was phosphate buffer and acetonitrile(84∶16),the flow rate was 1.0 mL/min,and the injection volume 10μL,the collection wavelength range is 190~400 nm,and the detection wavelength is 229 nm.The results showed that acetaminophen,analgin,antipyrine,and aminopyrine had good linearity in the concentration range of 1.17~117.28,2.03~202.92,5.58~111.60,2.42~120.8μg/mL,respectively.The correlation coefficients R 2 were 0.9986,0.9999,0.998,0.9993,and the average recovery rates(n=5)were 93.15%~104.25%,93.79%~104.62%,96.96%~99.17%,94.66%~99.83%,RSD They were 0.46%~2.29%,0.30%~3.15%,0.12%~0.94%,0.37%~1.42%(n=5).The method is convenient,fast,and accurate,and can be used for screening and testing four types of antipyretic and analgesic drugs illegally added to liquid preparations of veterinary traditional Chinese medicine. |
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| Keywords: | analgesic-antipyretic liquid preparation of chinese medicine Illegally added HPCL-PDA |
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