| 土施纳米氧化锌对蚯蚓生理和黄瓜幼苗生长的影响 |
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| 引用本文： | 杨静雅,符倩,张皓月,彭晴晴,钟民正,毛晖.土施纳米氧化锌对蚯蚓生理和黄瓜幼苗生长的影响[J].农业环境科学学报,2021,40(3):525-534. |
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| 作者姓名： | 杨静雅 符倩 张皓月 彭晴晴 钟民正 毛晖 |
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| 作者单位： | 农业农村部西北植物营养与农业环境重点实验室, 陕西 杨凌 712100;西北农林科技大学资源环境学院, 陕西 杨凌 712100 |
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| 基金项目： | 国家科技支撑计划项目（2015BAD23B04）；公益性行业（农业）科研专项（201503124）；国家自然科学基金项目（41571282）；中央高校基本科研业务费（2452015047） |
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| 摘 要： | 纳米氧化锌(ZnO NPs)由于其独特的理化性质,被广泛应用于各行各业,在带来巨大利益的同时也存在着潜在风险,如细胞毒性、基因毒性等。在土壤环境中,ZnO NPs可能会对陆地生态系统中的生物构成严重威胁。本研究采用盆栽试验的方法,评估了ZnO NPs对蚯蚓生理以及黄瓜幼苗生长的影响。结果表明:各处理ZnO NPs对蚯蚓的生长发育影响不显著,但随着浓度的增加,蚯蚓的抗氧化酶活性发生变化,SOD呈现出增加的趋势,CAT和POD先降低再上升,二者表现为协同作用。当ZnO NPs处理为1 000 mg·kg-1时,蚯蚓受到的氧化胁迫最大,MDA含量较对照增加了19.2%。对于黄瓜幼苗而言,ZnO NPs处理能够普遍增强植株氧化应激,除了SOD在1 000 mg·kg-1时受到抑制外,SOD、CAT、POD及MDA均随浓度的增加而增加。蚯蚓的加入能显著促进幼苗生长,增加生物量、提高根系活力,同时也能在一定程度上缓解或抑制ZnO NPs对植株造成的损伤。ZnO NPs处理为500mg·kg-1和1 000 mg·kg-1时,SOD分别较不加蚯蚓处理降低了76.7%、63.5%,MDA分别降低了28.6%、23.7%。在28 d培养周期中,蚯蚓对ZnO NPs响应较低,组织锌含量没有显著变化;黄瓜幼苗地上部和地下部锌含量均随着处理浓度的增加而增加,而添加蚯蚓的处理降低了植株的锌含量,也反映了蚯蚓在系统中对胁迫条件下的黄瓜幼苗生长的调控和缓解作用。
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| 关 键 词： | 纳米氧化锌 蚯蚓 黄瓜幼苗 氧化胁迫 锌含量 |
| 收稿时间： | 2020/9/15 0:00:00 |
Effects of zinc oxide nanoparticle application in soil on earthworm physiology and cucumber seedling growth |
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| YANG Jing-y,FU Qian,ZHANG Hao-yue,PENG Qing-qing,ZHONG Min-zheng,MAO Hui.Effects of zinc oxide nanoparticle application in soil on earthworm physiology and cucumber seedling growth[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2021,40(3):525-534. |
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| Authors: | YANG Jing-y FU Qian ZHANG Hao-yue PENG Qing-qing ZHONG Min-zheng MAO Hui |
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| Institution: | Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling 712100, China;College of Resources and Environmental Science, Northwest A&F University, Yangling 712100, China |
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| Abstract: | Zinc oxide nanoparticles(ZnO NPs) are widely used in various industries owing to their unique physical and chemical properties. While they have tremendous benefits, they also carry potential risks, such as cytotoxicity and genotoxicity. In the soil environment, ZnO NPs may pose a severe threat to organisms in terrestrial ecosystems. In this study, a pot experiment was used to evaluate the effects of ZnO NPs on earthworm physiology and cucumber seedling growth. The results showed that the ZnO NPs in each treatment had no significant effects on earthworms'' growth and development. However, as concentration increased, the antioxidant enzyme activity in earthworms was altered. SOD showed an increasing trend. CAT and POD first decreased and then increased, indicating a synergistic effect. When the ZnO NPs treatment was 1 000 mg·kg-1, earthworms suffered the most oxidative stress, and the MDA content increased by 19.2% compared with the control. For cucumber seedlings, ZnO NPs treatment exacerbated plant oxidative stress. Except that SOD was inhibited at 1 000 mg·kg-1, SOD, CAT, POD, and MDA all increased with an increase in ZnO NPs concentration. The addition of earthworms could significantly promote seedling growth, including increasing biomass and improving root vigor. Simultaneously, earthworms could alleviate or inhibit the damage caused by ZnO NPs to plants to a certain extent. When ZnO NPs were applied at 500 mg·kg-1 and 1 000 mg·kg-1, SOD was reduced by 76.7% and 63.5%, and MDA was reduced by 28.6% and 23.7%, respectively. During the 28-day culture cycle, the earthworms had a low response to ZnO NPs, and the zinc content in the earthworms tissues did not change significantly. The zinc content of above and belowground parts of cucumber seedlings increased with increasing treatment concentration. In contrast, the treatment combined with earthworms reduced the plants'' zinc content, reflecting the regulation and alleviation of cucumber seedling growth under stress conditions by earthworms. |
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| Keywords: | zinc oxide nanoparticles earthworms cucumber seedlings oxidative stress zinc content |
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