| 硫辛酸对草鱼脂肪细胞脂质含量及脂代谢相关基因表达的影响 |
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| 引用本文： | 黄陈翠,孙健,吉红,黄小城,边晨晨.硫辛酸对草鱼脂肪细胞脂质含量及脂代谢相关基因表达的影响[J].淡水渔业,2020(1):87-92. |
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| 作者姓名： | 黄陈翠 孙健 吉红 黄小城 边晨晨 |
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| 作者单位： | 西北农林科技大学动物科技学院水产动物营养与饲料研究室 |
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| 基金项目： | 陕西省科技统筹创新工程计划项目(2015KTTSNY01-05);陕西省科技厅人才计划项目(2018TD-021) |
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| 摘 要： | 为了探究硫辛酸对草鱼(Ctenopharyngodon idellus)脂肪细胞脂质代谢的影响,分离草鱼腹腔脂肪组织,消化得到脂肪细胞进行体外培养,分别用0、50和200μmol/L硫辛酸孵育细胞6 h,收取细胞和培养基样品后检测细胞甘油三酯(TG)含量、甘油和非酯化脂肪酸(NEFA)释放量及脂代谢相关基因表达。结果显示:50和200μmol/L硫辛酸处理显著降低草鱼脂肪细胞TG含量,200μmol/L硫辛酸显著增加细胞甘油和NEFA的释放量。200μmol/L硫辛酸组脂肪细胞脂肪分解相关基因(HSL、PPARα、CPT1、CD36)mRNA表达显著高于对照组,且ACC和PPARγ的mRNA表达水平显著上升,ATGL、MGL、SREBP-1c、FAS、DGAT等脂代谢基因表达状况无显著变化。综上所述,硫辛酸可能通过诱导草鱼脂肪细胞HSL和CPT1的基因表达促进脂质水解和β-氧化,从而降低脂肪细胞脂质含量。
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| 关 键 词： | 脂肪细胞 草鱼(Ctenopharyngodon idellus) 硫辛酸 脂代谢 |
Effect of α-lipoic acid on lipid content and lipid metabolism related gene expression in Ctenopharyngodon idellus adipocyte |
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| HUANG Chen-cui,SUN Jian,JI Hong,HUANG Xiao-chen,BIAN Chen-chen.Effect of α-lipoic acid on lipid content and lipid metabolism related gene expression in Ctenopharyngodon idellus adipocyte[J].Freshwater Fisheries,2020(1):87-92. |
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| Authors: | HUANG Chen-cui SUN Jian JI Hong HUANG Xiao-chen BIAN Chen-chen |
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| Institution: | (Laboratory of Aquatic Animal Nutrition and Feed Research,College of Animal Science and Technology,Northwest A&F University,Xianyang 712100,Shanxi,China) |
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| Abstract: | Adipocyte of Ctenopharyngodon idellus were obtained by digesting intraperitoneal adipose tissue and cultured in vitro to explore the effect ofα-lipoic acid on the lipid metabolism in adipocyte.Then adipocyte were treated with 0,50 and 200μmol/Lα-lipoic acid for 6 h,collected cells and medium samples to detect triglyceride(TG)content,the release of glycerol and non-esterified fatty acids(NEFA)content and lipid metabolism related genes expression.The present study results showed that 50 and 200μmol/Lα-lipoic acid treatment significantly decreased TG content in adipocyte of grass carp,and 200μmol/Lα-lipoic acid significantly increased the release of glycerol and NEFA content.The mRNA expression of lipid catabolism related genes(HSL,PPARα,CPT1 and CD36)in 200μmol/Lα-lipoic acid adipocyte group were significantly higher than that in control group.The mRNA expression of ACC and PPARγwere significantly increased by 200μmol/Lα-lipoic acid treatment.The mRNA expression of ATGL,MGL,SREBP-1c,FAS and DGAT had no significant change by 200μmol/Lα-lipoic acid treatment.In summary,α-lipoic acid could reduce lipid content by enhancing HSL and CPT1 genes expression to promote lipolysis andβ-oxidation in adipocyte of Ctenopharyngodon idellus. |
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| Keywords: | adipocyte Ctenopharyngodon idellus α-lipoic acid lipid metabolism |
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