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| 影响受体牛冷冻移植胚胎效果的主要因素 | |
| 引用本文： | 付静涛,朱士恩,李树静,刘国世,柳占奇.影响受体牛冷冻移植胚胎效果的主要因素[J].中国畜牧杂志,2008,44(17). |
| 作者姓名： | 付静涛 朱士恩 李树静 刘国世 柳占奇 |
| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193;北京市农业职业学院,北京,102442 2. 中国农业大学动物科技学院,北京,100193;农业生物技术国家重点实验室,北京,100193 3. 中国农业大学动物科技学院,北京,100193 4. 甘肃省酒泉市畜牧服务中心,甘肃酒泉,735000 |
| 基金项目： | 北京市科委科研项目，国家科技支撑计划 |
| 摘 要： | 试验研究影响受体牛移植冷冻胚胎效果的主要因素,目的是解决胚胎移植产业化中容易出现的技术问题,以提高牛胚胎移植妊娠率。在季节、饲养管理、同期发情处理和胚胎移植技术以及环境条件等基本相同的情况下,结果表明:西门塔尔杂交牛的移植利用率和移植妊娠率高于黄牛杂交牛和黑白花奶牛,但各组间无显著性差异(P>0.05);2～6周岁的受体牛移植利用率和移植妊娠率高于7周岁以上和2周岁以下的受体牛,但各组间无显著性差异(P>0.05);经产1～3胎受体牛移植利用率显著高于4胎以上的受体牛(P<0.05),而移植妊娠率则无显著性差异(P>0.05);体况上等与中等的受体牛移植利用率和移植妊娠率无显著性差异(P>0.05);同期发情处理时卵巢处于黄体期的移植利用率显著高于卵泡期,差异极显著(P<0.01);左侧卵巢有黄体的子宫角胚胎移植妊娠率显著高于右侧(P<0.05);胚龄对胚胎移植妊娠率高低依次为早期囊胚、桑椹胚和囊胚,但无显著性差异(P>0.05)。 |
| 关 键 词： | 冷冻胚胎 移植妊娠率 主要因素 受体牛 |
Effects of Recipient Cows on Transfer Efficiency of the Frozen-thawed Embryos |
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| FU Jing-tao,ZHU Shi-en,LI Shu-jing,LIU Guo-shi,LIU Zhan-qi.Effects of Recipient Cows on Transfer Efficiency of the Frozen-thawed Embryos[J].Chinese Journal of Animal Science,2008,44(17). | |
| Authors: | FU Jing-tao ZHU Shi-en LI Shu-jing LIU Guo-shi LIU Zhan-qi |
| Abstract: | The study was conducted at almost the same experimental and environmental conditions(such as season,breeding management,estrus synchronization and the technique of embryo transfer) to investigate the effects of recipient cows on transfer efficiency of the frozen-thawed embryos,solving some problems commonly existed in embryonic engineering industrialization.The results demonstrated that the percentage of available recipients or pregnancy rates in Simmental cross-bred cattle(or in recipients cows aged two to six years) were higher(P > 0.05) than those in indigenous cross-bred and holstein cows(or in recipients of younger than two years or older than seven years);that significant difference(P < 0.05) was observed in the rates of available recipients between one to three-parity cows and ≥four-parity cows,but the pregnancy rates between them was similar(P > 0.05);that there was no significant difference(P > 0.05) in the rate of available recipients or pregnancy rates between the cows in good and medium condition;that the rate of available recipients treated for estrus synchronization when ovum was in luteal stage was higher(P < 0.01) than that in follicular stage;that the pregnancy rates of embryos transferred into left uterus horn with corepus luteum was higher(P < 0.05) than that in right one;and that the pregnancy rates of early blastocysts were higher(P > 0.05) than that of morulae,which were higher(P > 0.05)than that of blastocysts. |
| Keywords: | cryopreservation pregnany main factors recipient cattle |
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