| 玉米种质材料遗传多样性的ISSR分析（英文） |
| |
| 作者姓名： | Vu Van Liet Nguyen Thi Thuy Linh Nguyen Thi Thuy Vu Thi Bich Hanh Pham Quang Tuan Nguyen Thi Phuong Thao |
| |
| 作者单位： | 河内农业大学水稻研究所;河内农业大学生物技术系; |
| |
| 摘 要： | 【目的】利用ISSR分子标记分析越南北部山区当地玉米种质材料的遗传多样性。【方法】采用ISSR技术和10个引物对从越南和老挝北部山区3个省收集的21份玉米种质材料即12份普通玉米和9份糯玉米的遗传多样性进行分析。【结果】在21份玉米种质材料中可以检测到108个ISSR片段,其多态性为100%。ISSR引物的多态性信息量值为0.10～0.39,每条引物平均为0.24;分辨力为14.29~0.48,平均每条为4.48。除ISSR-T1以外,所有ISSR引物在13份玉米材料中均产生特异性片段。根据聚类分析结果,使用70%的遗传相似性作为切割点,建立了玉米种质材料系统树,21份玉米种质材料被分为3大类。不同玉米材料的相似系数为0.52~0.90.【结论】ISSR标记可提供玉米种质遗传多样性信息,对越南玉米种质材料的收集、保护和育种具有重要作用。
|
| 关 键 词： | ISSR分子标记 遗传多样性 玉米地方品种 聚类分析 PCR |
Genetic diversity of maize(Zea mays L.) accessions using inter-simple sequence repeat(ISSR) markers |
| |
| Vu Van Liet,Nguyen Thi Thuy Linh,Nguyen Thi Thuy,Vu Thi Bich Hanh,Pham Quang Tuan,Nguyen Thi Phuong Thao.Genetic diversity of maize(Zea mays L.) accessions using inter-simple sequence repeat(ISSR) markers[J].Guangxi Agricultural Sciences,2011(9):1029-1034. |
| |
| Authors: | Vu Van Liet Nguyen Thi Thuy Linh Nguyen Thi Thuy Vu Thi Bich Hanh Pham Quang Tuan Nguyen Thi Phuong Thao |
| |
| Institution: | Vu Van Liet1,Nguyen Thi Thuy Linh2,Nguyen Thi Thuy2,Vu Thi Bich Hanh1,Pham Quang Tuan1,Nguyen Thi Phuong Thao2 (1Rice Research Institute,Hanoi University of Agriculture,Hanoi,Vietnam,2 Faculty of Biotechnology,Vietnam) |
| |
| Abstract: | 【Objective】The present study was conducted to analyze the characterization of the genetic diversity among local maize accessions in the North mountain region of Vietnam using ISSR markers.【Method】The ISSR technique and 10 primers were used to study genetic diversity among 21 maize accessions consisting of 12 normal and 9 waxy maize accessions, collected from 3 provinces in the North mountain region of Vietnam and Laos.【Result】A total of 108 ISSR fragments were detected and all of them were polymorphic（100%）.Polymorphism information content（PIC）values of ISSR primers ranged from 0.10-0.39.The average PIC value for each primer was 0.24.The resolving power（Rp）value ranged from 14.29- 0.48 with an average of 4.48 per primer.All of tested ISSR primers with the exception of ISSR-T1 generated unique frag- ments（present only in one accession）in 13 among 21 maize accessions.Based on UPGMA analysis,using 70%genetic similarity as the cutoff,a dendrogram was constructed and 21 maize accessions were grouped into three clusters.The simi larity coefficients among accessions ranged from 0.52-0.90.【Conclusion】ISSR markers provided information on genetic diversity of maize accessions which was useful for collection,conservation and breeding programs in Vietnam. |
| |
| Keywords: | ISSR markers genetic diversity maize landraces UPGMA analysis PCR |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
