| 鹅副粘病毒感染的诊断 |
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| 引用本文： | 钱晨,张建军.鹅副粘病毒感染的诊断[J].安徽农业科学,2007,35(36):11837-11838. |
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| 作者姓名： | 钱晨 张建军 |
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| 作者单位： | 扬州大学兽医学院,江苏扬州,225009;江苏省扬州威克生物工程有限公司,江苏扬州,225127 |
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| 摘 要： | 目的]为了确定江苏省某养鹅场2007年春季发生传染病的病因。方法]从发病鹅群采集病料进行流行病学调查、病原分离鉴定、红血球凝集试验和红血球凝集抑制试验,通过交叉HI试验测定F2代分离株和Lasota毒株的HI效价,并用稀释的F2代感染鸡胚尿囊液分别感染鹅和SPF雏鸡,观察其发病和死亡情况。结果]通过SPF鸡胚接种与传代从病、死鹅的脑、脾、胰混合病料分离到1株病毒。经HA和HI试验证明该分离株为禽I型副粘病毒。人工感染鸡和鹅试验表明该分离株对鸡和鹅都具有高致病性,同群混饲试验表明该分离株具有很强的传染性,属强毒型NDV。这进一步证实该病毒属禽副粘病毒的新成员,即鹅副粘病毒I型。结论]该研究为NDV出现了某些新的致病特点以及其对鹅同样具有致病性提供了佐证。
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| 关 键 词： | 鹅 病毒 HI 副粘病毒 |
| 文章编号： | 0517-6611（2007）36-11837-02 |
| 收稿时间： | 2007-11-13 |
| 修稿时间： | 2007年11月13 |
Diagnosis of Goose Paramyxovirus Infection |
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| QIAN Chen et al.Diagnosis of Goose Paramyxovirus Infection[J].Journal of Anhui Agricultural Sciences,2007,35(36):11837-11838. |
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| Authors: | QIAN Chen |
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| Abstract: | Objective] The research aimed to confirm the diseased cause of infection that occurred in a goose farm in Jiangsu province in Spring of 2007.Method] The diseased samples were collected from the diseased gaggle to make epidemiological investigation,pathogen isolation and identification,hemagglutination(HA) test and hemagglutination inhibition(HI) test.The HI titers of F2 isolates and Lasota virus strain were determined in cross HI test.The diluted allantoic fluid of F2 infected chicken embryo was used to infect geese and SPF chicken to observe the disease and death conditions.Result] By SPF chicken embryo inoculation and passage,one virus strain was isolated form the mixed diseased samples of brain,spleen and pancreas of diseased and dead geese.This isolated strain was proved to be avian paramyxovirus type I through HA and HI test.Artificial infection test on chicken and geese showed that this strain both had high pathogenicity to chicken and geese,and the test of mixed feeding in the same group showed that this isolate had stronger infectivity and it belonged to virulent NDV,which further validated that this virus was a new member of avian paramyxovirus,namely goose paramyxovirus.Conclusion] This research provided evidences for the occurrence of some new pathogenic characteristics of NDV and its pathogenicity to goose in the same way. |
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| Keywords: | Goose Virus HI Paramyxovirus |
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