| EST-SSR标记及其在果树研究中的应用 |
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| 引用本文： | 姚利华,滕元文.EST-SSR标记及其在果树研究中的应用[J].果树学报,2008,25(2):219-224. |
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| 作者姓名： | 姚利华 滕元文 |
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| 作者单位： | 浙江大学园艺系,农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029 |
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| 基金项目： | 浙江省自然科学基金杰出青年团队项目(R307605) |
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| 摘 要： | 近年来大量ESTs数据的出现已成为开发SSR标记的重要来源。EST-SSR作为一种新型的分子标记,不仅具备传统基因组SSR标记的特点,而且以其开发经济和通用性高等优势已在很多植物中得到开发和应用。简要介绍了EST-SSR标记的原理和特点,重点综述了其在果树的遗传多样性分析、遗传连锁图谱的构建、比较作图和分子系统发育等研究领域的应用现状,期望为今后该项技术在果树研究上的应用提供参考。
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| 关 键 词： | 果树 EST-SSR 遗传育种 遗传多样性分析 分子系统发育 |
| 文章编号： | 1009-9980（2008）02-219-05 |
| 收稿时间： | 2007-09-10 |
| 修稿时间： | 2007-11-27 |
A brief introduction on EST-SSR marker and its application in the research of fruit trees |
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| YAO Li-hua,TENG Yuan-wen.A brief introduction on EST-SSR marker and its application in the research of fruit trees[J].Journal of Fruit Science,2008,25(2):219-224. |
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| Authors: | YAO Li-hua TENG Yuan-wen |
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| Abstract: | Due to the availability of a vast amount of expressed sequence tags(ESTs),it has provided an important source for developing SSR marker.As a new kind of molecular marker,EST-SSR has been developed and used in many plant species because it has several advantages over genomic-derived SSRs,such as inexpensive development,high transferability,besides possessing the features of the latter one.Here,we give a brief introduction about the principles and characteristics of EST-SSR technique.Moreover,its application in some research fields of pomology,including genetic diversity analysis,genetic linkage map construction,comparative mapping and molecular phylogeny are reviewed.We hope this paper will be helpful for the future application of EST-SSR technique in the research of fruit trees. |
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| Keywords: | Fruit trees EST-SSR Genetic breeding Genetic diversity analysis Molecular phylogeny |
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