| 芥菜型油菜雄性不育系与甘蓝远缘杂交胚培养及早代育性鉴定 |
| |
| 引用本文： | 王涛涛,蔡晓峰,张俊红,李汉霞,叶志彪.芥菜型油菜雄性不育系与甘蓝远缘杂交胚培养及早代育性鉴定[J].园艺学报,2010,37(10):1661-1666. |
| |
| 作者姓名： | 王涛涛 蔡晓峰 张俊红 李汉霞 叶志彪 |
| |
| 作者单位： | （华中农业大学园艺植物生物学教育部重点实验室，华中农业大学园艺林学学院，武汉430070） |
| |
| 基金项目： | 国家'863'计划项目，国家公益性行业科技计划项目，华中农业大学科技创新基金，国家大学生创新性实验计划项目 |
| |
| 摘 要： | 为了将优异的芥菜型油菜雄性不育性转育到甘蓝中，对甘蓝和芥菜型油菜不育系远缘杂交胚的发育情况进行了研究，结果发现杂交胚发育10 d左右后开始败育。在杂交胚发生败育之前进行胚离体培养，在MS基本培养基中添加1 mg · L-1 BA，0.1 mg · L-1 NAA和活性炭的条件下，得到了远缘杂交胚发育而成的植株。进而利用石蜡切片对远缘杂交植株的不育性进行了显微观察，发现其败育的时期发生在雄蕊原基分化时期。
|
| 关 键 词： | 甘蓝 芥菜型油菜 雄性不育 胚培养 |
| 收稿时间： | 2010-06-13 |
The Culture and Early Male Sterile Identification of Distant Hybrid Embryos Derived from Brassica oleracea var.capitata L.and Male Sterile Line in B.juncea |
| |
| WANG Tao-tao,CAI Xiao-feng,ZHANG Jun-hong,LI Han-xia,YE Zhi-biao.The Culture and Early Male Sterile Identification of Distant Hybrid Embryos Derived from Brassica oleracea var.capitata L.and Male Sterile Line in B.juncea[J].Acta Horticulturae Sinica,2010,37(10):1661-1666. |
| |
| Authors: | WANG Tao-tao CAI Xiao-feng ZHANG Jun-hong LI Han-xia YE Zhi-biao |
| |
| Institution: | （Key Laboratory of Horticultural Plant Biology，Ministry of Education，Huazhong Agricultural University，College of Horticulture and Forestry，Huazhong Agricultural University，Wuhan 430070，China） |
| |
| Abstract: | The development of the distant hybrid embryos derived from Brassica oleracea var. capitata L. and male sterile line of B. juncea were investigated in this study. The results showed that the hybrid embryos began to abort in approximate 10 days after pollination. Some hybrid plantlets were obtained by using ovule rescue culture in vitro in MS media supplemented with 1 mg · L-1 BA，0.1 mg · L-1 NAA and activated charcoal. The development of anther in distant hybrid plants were examined using paraffin section，and the abortion was found in the differentiation stage of stamen primordium. |
| |
| Keywords: | Brassica juncea male sterile cabbage ovule rescue culture |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
