A magnetic nanoprobe technology for detecting molecular interactions in live cells |
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| Authors: | Won Jaejoon Kim Mina Yi Yong-Weon Kim Young Ho Jung Neoncheol Kim Tae Kook |
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| Institution: | Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea. |
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| Abstract: | Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins. |
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