| 甘蔗（Saccharum officinarum L.）品种遗传差异的AFLP分子标记分析 |
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| 引用本文： | 李鸣,谭裕模,李杨瑞,李容柏,高国庆.甘蔗（Saccharum officinarum L.）品种遗传差异的AFLP分子标记分析[J].作物学报,2004,30(10):1008-1013. |
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| 作者姓名： | 李鸣 谭裕模 李杨瑞 李容柏 高国庆 |
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| 作者单位： | 广西作物遗传改良生物技术重点开放实验室，广西南宁 530007 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 采用AFLP技术，从64对引物中筛选出5对带型分布均匀、多态性丰富且分辨能力强的引物，对两个在生产上广泛推广应用的甘蔗品种的AFLP指纹图谱进行分析，计算了两品种间的遗传相似性和遗传距离。结果表明，5对引物在2个甘蔗品种间均存在显著的差异，其中多态性位点占总扩增位点的10.2%，区分率达100%。这对应用AFLP技术鉴定甘蔗
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| 关 键 词： | 甘蔗（Saccharum officinarum L.） AFLP 指纹图谱 品种鉴定 |
| 收稿时间： | 2003-05-13 |
| 修稿时间： | 2003年5月13日 |
AFLP Molecular Analysis of Genetic Difference between Cultivars in Sugarcane(Saccharum officinarum L.) |
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| LI Ming ,TAN Yu-Mo,LI Yang-Rui ,,LI Rong-Bai,GAO Guo-Qing.AFLP Molecular Analysis of Genetic Difference between Cultivars in Sugarcane(Saccharum officinarum L.)[J].Acta Agronomica Sinica,2004,30(10):1008-1013. |
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| Authors: | LI Ming TAN Yu-Mo LI Yang-Rui LI Rong-Bai GAO Guo-Qing |
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| Institution: | Guangxi Crop Genetic Improvement and Biotechnology Laboratory，Nanning 530007, Guangxi |
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| Abstract: | Five pairs of AFLP primers from 64 pairs were selected, which had rich polymorphism. The genetic diversity of two sugarcane cultivars was analyzed with the fingerprinting mapped by the 5 primer pairs,and the genetic similarity and distance were estimated. The results showed that there were distinct differences between the two cultivars. The rate of the polymorphism bands was 10.2%,and the rate of identification was 100%.The key techniques of AFLP analysis system applied in sugarcane,the reliability and safety of silver staining were also discussed. |
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| Keywords: | AFLP |
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