Application of isozyme electrophoresis for purity testing and cultivar identification of F1 hybrids of Brassica oleracea |
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| Authors: | P Arús C R Shields T J Orton |
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| Institution: | (1) Servei d'Investigació Agrària, Carretera de Cabrils s/n, Cabrils, (Barcelona), Spain;(2) Department of Agronomy and Range Science, University of California, 95616 Davis, CA, USA;(3) Applied Genetics Laboratory, Agrigenetics Corporation, 3375 Mitchell Lane, 80301 Boulder, CO, USA |
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| Abstract: | Summary Six isozyme genes were analyzed in seed samples of 65 commercial F1 hybrids of four horticultural groups of Brassica oleracea (cabbage, Brussels sprouts, sprouting broccoli and cauliflower). Results obtained from electrophoretic assays led to the following conclusions: 1) the electrophoretic test of F1 hybrid purity was possible in 59 (91%) of the hybrids analyzed, since their inbred parents were apparently fixed each for a different allele in at least on of the loci studied; 2) forty-eight (74%) of the hybrids were individually distinguished by their isozyme phenotype; 3) high levels of segregation in the inbred parents were inferred from the analysis of a sample of seeds of each hybrid. |
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| Keywords: | Brassica oleracea cabbage Brussels sprouts sprouting broccoli cauliflower isozymes F1 hybrid purity testing cultivar identification |
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