| 乳铁蛋白的分离工艺研究 |
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| 引用本文： | 吕立获,周晓云,姚婷婷,芦国营,吴绵斌.乳铁蛋白的分离工艺研究[J].饲料工业,2005,26(17):11-14. |
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| 作者姓名： | 吕立获 周晓云 姚婷婷 芦国营 吴绵斌 |
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| 作者单位： | 浙江工业大学生物与环境学院,310014,浙江工业大学研35# |
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| 摘 要： | 乳铁蛋白是哺乳动物乳中的生物活性物质,研究讨论了从牛初乳中分离乳铁蛋白的工艺。牛初乳经过离心脱脂得到脱脂乳,加入带羧甲基的弱酸性阳离子交换剂CM-SepharoseFastFlow在4℃下进行动态吸附,用去离子水将没有吸附上的杂蛋白冲洗掉,然后使用1.6%和5%的NaCl溶液进行阶跃洗脱;经过两步阶跃洗脱得到的乳铁蛋白纯度可达95%以上。
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| 关 键 词： | 牛初乳 乳铁蛋白 离子交换层析 |
| 收稿时间： | 2005-07-27 |
| 修稿时间： | 2005年7月27日 |
Study on Separating Process of Lactoferrin |
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| Lv LiHuo;Zhou XiaoYun;Yao TingTing;Lu GuoYing;Wu MianBin.Study on Separating Process of Lactoferrin[J].Feed Industry,2005,26(17):11-14. |
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| Authors: | Lv LiHuo;Zhou XiaoYun;Yao TingTing;Lu GuoYing;Wu MianBin |
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| Abstract: | The Lactoferrin is the living beings active material in the mammal mamma , and the original mainly talked about the technology of Lactoferrin departs from bovine colostrum: The bovine colostrum obtains the skimmed milk after being at odds with the community to degrease, and accedes to brining Qiang's methyl weak acid cation exchange dose a CM - Sepharose Fast Flow being living to carry on static state below 4℃s adsorbing; In the way of leave ion water shall not there is, and sundry albumen on the sticking rinses, afterwards employ the NaCl's liquor of 1.5% and 5% to carry on the stairs and jumps to wash comeing off ; After two jumps wash to come off over the Lactoferrin pureness attainability 95% of mamma that obtains. |
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| Keywords: | bovine colostrum Lactoferrin cation-exchange adsoption |
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