| 野生湖北百合不定芽诱导及再生植株的建立 |
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| 引用本文： | 蒋瑶,陈文波,周朝伟,陈其兵.野生湖北百合不定芽诱导及再生植株的建立[J].黑龙江农业科学,2016(9):11-14. |
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| 作者姓名： | 蒋瑶 陈文波 周朝伟 陈其兵 |
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| 作者单位： | 1. 凯里学院环境与生命科学学院,贵州 凯里,556011;2. 四川农业大学风景园林学院,四川成都,611130 |
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| 基金项目： | 贵州省科技厅资助项目(黔科合 J 字[2014]2153号)；凯里学院博士启动基金资助项目(BS201332) |
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| 摘 要： | 为了加强野生湖北百合资源保存与利用,选用黔东南州野生湖北百合为材料,鳞茎及茎段为外植体,探讨不同浓度激素配比对其不定芽诱导、增殖及生根的影响。结果表明:诱导不定芽的最佳培养基为MS+1.0mg·L~(-1) 6-BA+0.5mg·L~(-1) NAA,但茎段诱导不定芽高于鳞片,出芽率分别为66.67%和59.63%,单块外植体出芽个数分别为2和1,且茎段污染率比鳞片的污染率低。茎段诱导最佳增殖培养基为MS+0.1mg·L~(-1) 6-BA+1.0mg·L~(-1) NAA,增殖率为83.33%,平均增殖系数为2.11,其生长状况良好,为最佳增殖培养基。茎段诱导最佳生根培养基为1/2MS+0.3mg·L~(-1) NAA,诱导生根率高达93.33%,平均生根数为3.18。
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| 关 键 词： | 湖北百合 鳞片 茎段 组织培养 |
Study on Adventitious Buds Induction and Plant Regeneration of Wild Liliumhenry i |
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| Abstract: | In order to strengthen the preservation and utilization of wild lilyresources in Hubei province ,using bulb and stem of wild Liliumhenryias explant ,the effect of different hormone combination on adventitious buds induction ,bud proliferation and rooting were studied .The results showed that the optimal medium for adventi‐tious buds induction was MS+1 .0 mg·L‐1 6‐BA+0 .5 mg·L‐1 NAA .The induction rate of stem was higher than bulbs .The rate of induction was 66 .67% and 59 .63% .The bud from single explant was 2 and 1 ,the rate of contamination from stem was lower than bulb .The best proliferation medium was MS + 0 .1 mg·L‐1 6‐BA+1 .0 mg·L‐1 NAA .The rate of proliferation was 83 .33% ,the average of coefficient of proliferation was 2 .11 and the growth was the better .The medium for rooting was 1/2MS+0 .3 mg·L‐1 NAA .The rate of roo‐ting was as high as 93 .33% ,the average number of rooting was 3 .18 . |
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| Keywords: | Liliumhenryi bulb stem tissue culture |
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