Towards a DNA marker assisted seed source identification: a pilot study in European beech (<Emphasis Type="Italic">Fagus sylvatica</Emphasis> L.) |
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| Authors: | N Hasenkamp B Ziegenhagen C Mengel L Schulze H-P Schmitt S Liepelt |
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| Institution: | 1.Faculty of Biology, Conservation Biology,University of Marburg,Marburg,Germany;2.Landesbetrieb Wald und Holz, NRW, Au?enstelle Arnsberg-Obereimer; ?kologischer Waldbau und Forstgenetik,Arnsberg,Germany |
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| Abstract: | The widespread European forest tree Fagus sylvatica L. is of great importance for forest management. However, information about seed dispersal is still very rare, though important
for harvesting strategies and later on seed source identification. We refined a DNA fingerprinting method for beech nut shells
in order to directly assign dispersed seeds to their mother trees. A pilot study was conducted in two beech stands in Germany
where leaves of the adult trees and the exocarp of dispersed seeds were fingerprinted at six nSSR loci. While one stand was
randomly analysed for adults and dispersed seeds the other was systematically investigated following common harvesting procedures.
Imitating the typical net harvesting strategy, seeds were collected beneath 19 adult trees. Exocarp genotyping revealed that
on average three different mother trees contributed to a sample of five or six seeds collected beneath a single adult tree.
Of the identified mother trees most were located within a radius of 15 m from the sampling point. The repeated pattern of
seed dispersal within a short distance constitutes the basis for a straightforward strategy for the assignment of seed lots
to a seed source stand. This strategy is based on the matching of individual genotypes without the necessity for a full inventory
of the putative source stand. Additionally, we provide allelic ladders of five nSSR loci for standardization among laboratories. |
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