| 山核桃基因组DNA提取方法的比较研究 |
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| 引用本文： | 郭传友,黄坚钦,王正加.山核桃基因组DNA提取方法的比较研究[J].福建林业科技,2004,31(2):12-15. |
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| 作者姓名： | 郭传友 黄坚钦 王正加 |
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| 作者单位： | 1. 南京林业大学森林资源与环境学院,江苏,南京,210037
2. 浙江林学院生命科学系,浙江,临安,311300 |
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| 基金项目： | 浙江省科技厅重大项目(0211025)，浙江省自然科学基金重大项目(ZA0208)资助 |
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| 摘 要： | 植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响TaqDNA聚合酶的活性,是导致PCR扩增反应失败的主要因素。以山核桃嫩叶为材料,对分别用经过改良的SDS法、改良的CTAB法、简易CTAB法及改良的高盐低pH法提取的基因组DNA进行检测比较,结果显示,改良的SDS法更适合于山核桃基因组DNA的提取,此方法提取的DNA能很好地用于PCR扩增。
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| 关 键 词： | 山核桃 基因组DNA 提取方法 PCR扩增 |
| 文章编号： | 1002-7351(2004)02-0012-04 |
Study on Isolation Methods of Genomic DNA in Carya Cathayensis |
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| GUO Chuan-you,HUANG Jian-qin,WANG Zheng-jia.Study on Isolation Methods of Genomic DNA in Carya Cathayensis[J].Journal of Fujian Forestry Science and Technology,2004,31(2):12-15. |
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| Authors: | GUO Chuan-you HUANG Jian-qin WANG Zheng-jia |
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| Institution: | GUO Chuan-you~1,HUANG Jian-qin~2,WANG Zheng-jia~2 |
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| Abstract: | High content of protein,phenol,tannin,pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.The methods of improved CTAB,rapid CTAB,improved SDS and improved low pH medium were used for genomic DNA isolation in Carya cathayensis.The results indicated that the method of improved SDS was the most suitable one for Carya cathayensis genomic DNA isolation and the DNA isolated can be used for PCR reaction. |
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| Keywords: | Carya cathayensis genomic DNA isolation method PCR reaction |
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