| 甘蓝型油菜核不育基因的PCR标记初报 |
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| 引用本文： | 蒋梁材,
蒲晓斌,
王瑞,
张启行,
蔡平钟,.甘蓝型油菜核不育基因的PCR标记初报[J].西南农业大学学报,2000,22(3):199-202. |
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| 作者姓名： | 蒋梁材 蒲晓斌 王瑞 张启行 蔡平钟 |
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| 作者单位： | [1]四川省农科院作物所,成都 [2]四川省生物技术中心,成都 |
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| 基金项目： | “九五”国家品资攻关项目! (96 - 0 14- 0 1- 0 4 )，四川省生物技术育种项目 |
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| 摘 要： | 用Ｏｐｅｒｏｎ公司ＯＰＡ－０１－ＯＰＪ－２０ ２００个１０个碱基对随机序列引物对甘蓝型油菜隐性核不育涪优ＡＢ，显性核不育３７ＡＢ，广恢系９８－８０２６，９８－３７４９及Ｆ１材料基因组ＤＮＡ进行ＲＡＰＤ扩增的结果，共获得１３３４条扩增带，平均每引物扩增带约６．７条扩增带表明油菜存在较为丰富的遗传多样性。引物ＯＰＡ－０５，ＯＰＡ－１２，ＯＰＡ－１３，ＯＰＡ－２０等分别扩增出了大小不同的多态性带，初步认
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| 关 键 词： | 甘蓝型油菜 遗传多样性 核不育基因 PCR标记 |
PRELIMINARY STUDIES ON PCR MARKERS FOR GENETIC MALE STERILITY IN BRASSICA NAPUS L. |
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| JIANG Liang-cai,PU Xiao-bin,WANG Rui,ZHANG Qi-xin,CAI Ping-zhong.PRELIMINARY STUDIES ON PCR MARKERS FOR GENETIC MALE STERILITY IN BRASSICA NAPUS L.[J].Journal of Southwest Agricultural University,2000,22(3):199-202. |
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| Authors: | JIANG Liang-cai PU Xiao-bin WANG Rui ZHANG Qi-xin CAI Ping-zhong |
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| Institution: | JIANG Liang-cai 1,PU Xiao-bin 1,WANG Rui 1,ZHANG Qi-xin 1,CAI Ping-zhong 2 |
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| Abstract: | Two hundred random 10 bp-primers from Operon company were used in RAPD amplification of genome DNAs of the dominant genetic male sterile line 37AB, the recessive genetic male sterile line Fuyou AB, the poly-restorer lines 98-8206 and 98-3749 and their F 1s. A total of 1334 amplified bands were obtained, averaging 6.7 bands per primer, suggesting that abundant genetic plymorphisms exist in Brassica napus L. Primers OPA-05, OPA-09, OPA-12, OPA-13 and OPA-20 amplified several bands of different milecular weight. OPA-12 950 was the marker for the dominant genetic male sterile gene of 37A, and OPA-20 750 the marker for the recessive genetic male sterile gene of Fuyou A. OPA-05 1500 and OPA-13 3550 were shown to be related to the genetic male sterile genes. OPA-13 2300 was the characteristic band in B.napus. |
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| Keywords: | Brassica napus L genetic polymorphism RAPD molecular marker |
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