| 西番莲胚乳愈伤组织诱导和三倍体植株再生 |
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| 引用本文： | 张琴,
闫勇,
梁国鲁,.西番莲胚乳愈伤组织诱导和三倍体植株再生[J].西南农业大学学报,2000,22(5):398-402. |
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| 作者姓名： | 张琴 闫勇 梁国鲁 |
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| 作者单位： | 西南农业大学生物技术中心，重庆400716 |
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| 摘 要： | 用紫果西番莲种子的胚乳作外植体离体培养，在MS＋BA0．2mg/L NAA2.0mg/L培养基上诱导获得乳白色或淡黄色结构致密的愈伤组织，诱导频率达到84．7％。愈伤组织在MS＋BA2mg/L NAA0．2mg/L上分化不定芽的频率最高，达65％，增殖系数达到5．2。再生的不定芽在1／2MS＋IBA1mg/L上诱导生根的效果好，生根率达63％。再生植株经根尖染色体计数有95．3％的细胞染色体数目为2n=3x=27，证明再生植株为三倍体。说明胚乳培养是获得三倍体的高效途径。
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| 关 键 词： | 西番莲 胚乳培养 三倍体植株再生 愈伤组织 诱导 |
CALLUS INDUCTION AND TRIPLOID PLANT REGENERATION FROM ENDOSPERM OF PASSIFLORA EDULIS |
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| ZHANG Qin,YAN Yong,LIANG Guo-lu.CALLUS INDUCTION AND TRIPLOID PLANT REGENERATION FROM ENDOSPERM OF PASSIFLORA EDULIS[J].Journal of Southwest Agricultural University,2000,22(5):398-402. |
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| Authors: | ZHANG Qin YAN Yong LIANG Guo-lu |
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| Institution: | ZHANG Qin,YAN Yong,LIANG Guo-lu 1 * |
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| Abstract: | Endosperm of Passiflora edulis was used as explant. The callus induction rate (84.7%) was obtained when endosperm was cultured on MS supplemented with 0.2mg/L BA and 2.0 mg/L NAA.The highest shoot induction rate(65%)was abtained when callus was cultured on MS BA 2.0 mg/L NAA 0.2 mg/L and multiplication coefficient was 5.2. The shoots rooted readily on 1/2 MS supplemented with 1mg/L IBA and the root rate was 63%.The regenerated plantlets proved to be triploidy(2n=3x=27). |
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| Keywords: | Passiflora edulis Endosperm culture Triploid planL |
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