| 栉孔扇贝一种球形病毒的分离纯化及其超微结构观察 |
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| 引用本文： | 王崇明.栉孔扇贝一种球形病毒的分离纯化及其超微结构观察[J].水产学报,2002,26(2):180-184. |
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| 作者姓名： | 王崇明 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所农业部海洋渔业资源,可持续利用重点开放实验室,山东,青岛,266071
2. 青岛海洋大学水产学院,山东,青岛,266003 |
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| 基金项目： | 国家重点基础研究规划项目课题资助 (G19990 12 0 0 1) |
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| 摘 要： | 栉孔扇贝是我国北方沿海贝类养殖的重要经济种类 ,目前已成为我国北方海洋经济的支柱性产业之一。但随着近年来病害的不断加剧 ,养殖期栉孔扇贝的大规模死亡现象频繁发生 ,极大地威胁着这一产业的可持续发展。因此 ,国内围绕扇贝大规模死亡的致病因子及病原学的探讨已引起养殖界各方的高度关注1-4] 。2 0 0 0年我们对山东省内主要扇贝养殖海区开展了系统的病原学和流行病学调查。通过病贝组织超薄切片的电镜观察 ,在病害高发期的病贝体内大量发现一种球形病毒样颗粒。在此基础上 ,我们进一步完成了该病毒粒子的分离和纯化 ,现将有关结果报…
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| 关 键 词： | 栉孔扇贝 非包涵体 球形病毒 分离纯化 电镜观察 超微结构 支柱性产业 病毒粒子 |
| 文章编号： | 1000-0615(2002)02-0180-05 |
| 收稿时间： | 2014/4/10 0:00:00 |
| 修稿时间： | 2001年9月7日 |
Purification and ultrastructure of a spherical virus in cultured scallop Chlamys farreri |
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| WANG Chong ming.Purification and ultrastructure of a spherical virus in cultured scallop Chlamys farreri[J].Journal of Fisheries of China,2002,26(2):180-184. |
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| Authors: | WANG Chong ming |
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| Institution: | WANG Chong ming 1,WANG Xiu hua 1,SONG Xiao ling 1,HUANG Jie 1,SONG Wei bo 2 |
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| Abstract: | Electron microscopic examinations of ultrathin sections reveal the presence of spherical virus like particles in the digestive gland, kidney, and the intestine of moribund scallop Chlamys farreri collected during a severe mortality in Jiaozhou Bay (Qingdao, China) in 2000. The virions are approximately 130 to 170 nm in diameter and have a bilaminal envelope, while the nucleocapsids are 90-140 nm. The virus occurs and distributes in the cytoplasmic vesicles of infected cells, and it is arranged in a scattered form without occluding protein. The viral particles are consistent in size and morphology in preparations derived from the isolation and also from ultrathin sections. The spikes of envelope are clearly observed with negative staining. By the same methods, this virus was not found in the healthy scallop, indicating that this virus might be the causative aetiology for the abnormal mortality. |
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| Keywords: | Chlamys farreri non occluded spherical viruses isolation and purification electronic observation |
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