| 仔猪抗E2抗体水平的调查研究 |
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| 引用本文： | 吴其彪,田娟娟,谭春萍,黄百花,刘文娟,陆芹章.仔猪抗E2抗体水平的调查研究[J].广西农业科学,2011,42(1):98-101. |
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| 作者姓名： | 吴其彪 田娟娟 谭春萍 黄百花 刘文娟 陆芹章 |
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| 作者单位： | 广西大学动物科学技术学院,南宁,530005 |
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| 摘 要： | 【目的】寻找一种更具针对性、更为可靠的仔猪猪瘟抗体水平检测方法。【方法】分别采用间接ELISA、间接血凝试验（IHA），对广西某猪场的90份已超免的不同阶段仔猪血清进行抗猪瘟E2抗体和猪瘟全抗体水平监测。【结果】在间接ELISA试验中，阳性样品数为86份，占总样品比例的95．56％；间接血凝试验结果效价在1：16以上（含1：16）的血清样品为88份，占总样品比例的97．78％，两者差异不显著。【结论】仔猪血清中确实存在具有保护性的抗E2抗体，以间接ELISA检测抗E2抗体来监测猪瘟免疫水平是可行的，这为猪瘟免疫水平的监测提供了一种新方法，也为制定免疫程序提供了科学依据。
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| 关 键 词： | 仔猪 猪瘟 抗E2抗体 间接ELISA 间接血凝试验（IHA） |
Monitoring of classical swine fever virus E2 protein antibody levels in weaned piglets |
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| WU Qi-biao,TIAN Juan-juan,TAN Chun-ping,HUANG Bai-hua,LIU Wen-juan,LU Qin-zhang.Monitoring of classical swine fever virus E2 protein antibody levels in weaned piglets[J].Guangxi Agricultural Sciences,2011,42(1):98-101. |
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| Authors: | WU Qi-biao TIAN Juan-juan TAN Chun-ping HUANG Bai-hua LIU Wen-juan LU Qin-zhang |
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| Institution: | (College of Animal Science and Veterinary Medicine, Guangxi University, Nanning 530005, China) |
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| Abstract: | Objective]The present experiment was conducted to develop a direct and reliable detection method for classical swine fever （CSF） antibody level of weaned piglets. Method]Ninety serum samples were collected from weaned piglets of different ages from a swine piggery in Guangxi. The piglets were immunized before sucking colostrums. All the samples were monitored for E2 antibody and classical swine fever virus （CSFV） antibody using indirect enzyme-linked immuno sorbent assay （ELISA） and indirect hemagglutination （IHA） test. Result]Out of 90 samples, 86, accounting for 95.56% of the total samples, showed positive results to indirect ELISA test. The antibody titer of 88 serum samples was above 1：16 （including 1：16） in IHA test, which accounted for 97.78% of the total samples. Conclusion]The results indicated that the serum of piglets had protective anti-E2 antibody, which was found to be available for monitoring the CSFV immunization level using detection of serum antibody level of piglets using indirect ELISA. |
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| Keywords: | piglet classical swine fever anti-E2 antibody indirect ELISA IHA |
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