| 单双液相下不同多酚氧化酶源对酶性合成茶黄素的影响 |
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| 引用本文： | 谷记平,刘仲华,黄建安,施兆鹏,王桂雪.单双液相下不同多酚氧化酶源对酶性合成茶黄素的影响[J].茶叶科学,2007,27(1):76-82. |
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| 作者姓名： | 谷记平 刘仲华 黄建安 施兆鹏 王桂雪 |
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| 作者单位： | 教育部茶学重点实验室,湖南省天然产物工程技术研究中心,湖南,长沙,410128;中国农业科学院茶叶研究所,浙江,杭州,310008;教育部茶学重点实验室,湖南省天然产物工程技术研究中心,湖南,长沙,410128 |
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| 基金项目： | 科技部科技攻关项目
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湖南省科技厅科技计划 |
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| 摘 要： | 比较了单双液相条件下不同来源的多酚氧化酶对酶性合成茶黄素效率的影响。结果表明,在本试验体系下单双液相试验中,以不同的酶源合成茶黄素的试验效果不同,就茶鲜叶酶源而言,双液相明显比单液相的效果要显著,在单液相系统中总乙酸乙酯层茶黄素总含量达33.74%,合成率为14.245%,而在双液相系统中,其总含量达39.74%,合成率为31.792%;而就梨酶源处理而言,单液相的效果要比双液相稍好,在单液相系统中总乙酸乙酯层茶黄素总含量达45.73%,合成率为18.799%;而在双液相系统中,其总含量达40.20%,合成率为22.11%;就苹果酶源而言,单液相比双液相的效果要好些,在单液相系统中总乙酸乙酯层茶黄素总含量达18.22%,合成率为13.34%;而在双液相系统中,其总含量达11.56%,合成率为6.935%。
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| 关 键 词： | 茶黄素 双液相 单液相 酶源 |
| 文章编号： | 1000-369X（2007）01-076-07 |
| 收稿时间： | 2006-06-08 |
| 修稿时间： | 2006年6月8日 |
Influence of Different Enzyme Origin on the Enzyme-Catalysing Synthesizing of Theaflavins in Single and Bi-liquid Phases Systems |
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| GU Ji-ping,LIU Zhong-hua,HUANG Jian-an,SHI Zhao-peng,WANG Gui-xue.Influence of Different Enzyme Origin on the Enzyme-Catalysing Synthesizing of Theaflavins in Single and Bi-liquid Phases Systems[J].Journal of Tea Science,2007,27(1):76-82. |
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| Authors: | GU Ji-ping LIU Zhong-hua HUANG Jian-an SHI Zhao-peng WANG Gui-xue |
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| Institution: | 1. Key Laboratory of Tea Science of National Ministry of Education, Hunan province Engineering Research Center for Nature Prodcuts Changsha 410128, China;2. Tea Research Institute, Chienese Academy of Agricultural Sciences, Hangzhou 310008, China |
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| Abstract: | Synthesizing theaflavins (TFs) in single and bi-liquid phase systems was studied by various source of PPOs. The results showed that different effects could be gained in synthesizing the theaflavins by using the various source of PPOs. As to PPO from fresh tea leaves, the effect in bi-liquid phase system was more remarkable than that in single liquid phase system. The content of TFs in ethyl acetate layer in single liquid phase system, amounted at 33.74%, the synthesizing rate of TFs was 14.245%, while in bi-liquid phases system tests, the content of TFs in ethyl acetate layer was 39.74%, and the synthesizing rate of TFs was 31.792%. When the PPO was from pear, the effect in bi-liquid phase was slightly better than that in single liquid phase, the content of TFs in ethyl acetate layer, in single liquid phase system, amounted at 45.73%, the synthesizing rate of TFs was 18.799%, while in bi-liquid phases system, the content of TFs in ethyl acetate layer was 40.20%, and the synthesizing rate of TFs was 22.11%. However, when the PPO from apple, opposite results was gained that the effect in single liquid phase was better than that in bi-liquid phase, the content of TFs in ethyl acetate layer, in single liquid phase system, amounted at 18.22%, the synthesizing rate of TFs was 13.34%, while in bi-liquid phases system, the content of TFs in ethyl acetate layer was 11.56%, and the synthesizing rate of TFs was 6.935%. |
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| Keywords: | theaflavins(TFs) single-liquid phase bi-liquid phase enzyme-sources |
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