| 成熟脂肪细胞中脂联素基因表达的脂肪酸应答调控 |
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| 引用本文： | 袁继红,龙欢,雷霆,陈小冬,杨在清.成熟脂肪细胞中脂联素基因表达的脂肪酸应答调控[J].华中农业大学学报,2009,28(3). |
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| 作者姓名： | 袁继红 龙欢 雷霆 陈小冬 杨在清 |
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| 作者单位： | 华中农业大学生命科学技术学院,武汉,430070 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家高技术研究发展计划(863计划)，国家自然科学基金 |
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| 摘 要： | 用不同种类、不同浓度游离脂肪酸(FFA)处理体外培养的成熟3T3-L1脂肪细胞,实时荧光定量PCR和Western-blotting分析FFA处理前后脂联素基因mRNA和蛋白质水平的表达差异.结果表明:各类脂肪酸埘脂联素mRNA的表达均具有下调作用.且抑制效应呈时间、剂量依赖性;并且各种脂肪酸显著地影响脂联素蛋白的分泌作用.
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| 关 键 词： | 脂肪酸 脂联素 基因表达 脂肪细胞 |
Response of Fatty Acids to Adiponectin Expression in Matured Adipocytes |
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| YUAN ji-hong,LONG Huan,LEI Ting,CHEN Xiao-dong,YANG Zai-qing.Response of Fatty Acids to Adiponectin Expression in Matured Adipocytes[J].Journal of Huazhong Agricultural University,2009,28(3). |
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| Authors: | YUAN ji-hong LONG Huan LEI Ting CHEN Xiao-dong YANG Zai-qing |
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| Institution: | College of Life Science and Technology;Huazhong Agricultural University;Wuhan 430070;China |
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| Abstract: | 3T3-L1 preadipocytes were cultured and differentiated into the matured adipocytes in vitro.The differentiated adipocytes were incubated with different types of free fatty acids at different concentrations.Real-time quantitative PCR and Western-blotting were used to detect the expression of adiponectin mRNA and protein,respectively.The results showed that all types of fatty acids inhibitted adiponectin mRNA expression in dose and time-dependent manner.And all the fatty acids significantly influenced the secr... |
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| Keywords: | fatty acids adiponectin gene expression adipocytes |
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