Wheat endosperm cell walls: Spatial heterogeneity of polysaccharide structure and composition using micro-scale enzymatic fingerprinting and FT-IR microspectroscopy |
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| Authors: | Luc Saulnier Paul Robert Mathilde Grintchenko Frédéric Jamme Brigitte Bouchet Fabienne Guillon |
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| Institution: | 1. INRA, UR1268 Biopolymers, Interactions Assemblies, BP 71627, F-44316 Nantes, France;2. Synchrotron SOLEIL, L''orme des Merisiers, BP 48, Saint Aubin, F-91192 Gif sur Yvette, France |
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| Abstract: | Micro-scale enzymatic fingerprinting and FT-IR microspectroscopy were used to investigate changes of polysaccharide structure and composition in cell walls from wheat endosperm. These methods were applied to transverse and longitudinal sections of wheat grains harvested at maturity and 270°D. Principal component analysis treatment of the data revealed marked differences in the (1,3)-(1,4)-beta-glucans (BG)/arabinoxylans (AX) ratio and in the structure of AX depending on both cell position in the grain and stage of development. Cell walls close to the germ were enriched in BG in both developing and mature grain. AX in developing grain were characterized by a higher proportion of di-substitution by arabinose as compared to mature grain AX. In addition, AX in mature grains exhibited a different structure depending on prismatic or central origin of cell walls in the grain. These results indicated a high spatial and temporal control of the biosynthesis of AX and BG in wheat endosperm. |
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| Keywords: | Arabinoxylans (1 3)-(1 4)-beta-glucans Cereal Grain Endoxylanase Lichenase |
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