| 猪伪狂犬病病毒粤A株的分离与鉴定 |
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| 引用本文： | 刘镇明,蓝天,罗满林,袁少华,石迎新.猪伪狂犬病病毒粤A株的分离与鉴定[J].华南农业大学学报,2000,21(2):76-78. |
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| 作者姓名： | 刘镇明 蓝天 罗满林 袁少华 石迎新 |
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| 作者单位： | 华南农业大学动物医学系,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金资助项目! (39770 0 33) |
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| 摘 要： | 从广东番禺某猪场病死仔猪中分离到一株疑为猪伪狂犬病病毒（ＰＲＶ）毒株，通过对其理化特性的鉴定，中和试验，实验动物回归试验，电镜观察，ＰＣＲ扩增及感染力测定，发现其对氯仿、乙醚均敏感，能中和Ｐｒ不标准阳性血清，实验兔接种该病毒后，发生瘙痒并麻痹致死，电中见病毒粒子呈示规则圆形并带有囊和纤窝。ＰＣＲ体外扩增可见其不Ａ株在同一水平位置上有相同的电泳带，用Ｍａｒｃ１４５测定其毒为１０＾７．５ＴＣＩＤ５０／
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| 关 键 词： | 猪伪狂犬病病毒 中和试验 PCR检测 病原鉴定 |
| 修稿时间： | 1999-10-08 |
Isolation and Identification of Yue A Strain of Pseudorabies Virus |
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| LIU Zhen-ming,LAN Tian,LUO Man-lin,YUAN Shao-hu,SHI Ying-xin.Isolation and Identification of Yue A Strain of Pseudorabies Virus[J].Journal of South China Agricultural University,2000,21(2):76-78. |
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| Authors: | LIU Zhen-ming LAN Tian LUO Man-lin YUAN Shao-hu SHI Ying-xin |
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| Abstract: | An isolate suspected to be pseudorabies virus was recovered from dead piglets on a pig farm in Panyu Guangdong. It was characterized by its physicochemical properties, virus neutralization test, animal inoculation, electron microscopy, PCR amplification and in vitro infectivity test. The isolate was found to be sensitive both to chloroform and ether, could be neutralized by the standard pseudorabies Fujian A strain virus antibodies, rabbits inoculated with the isolate developed localized itch and then paralysis before death, electron microscope revealed irregularly round virus particles with envelope and peplomers, PCR amplification of the virus genome yielded a fragment which co migrated with similarly amplified fragment of the Fujian A standard PRV. When titrated in Marc 145 cell culture, the virus titre attained 10 7.5 TCID 50 /mL. The isolate was identified as a pseudorabies virus on the strength of the above findings. |
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| Keywords: | Pseudorabies virus (PRV) neutralization test PCR |
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