Antioxidant and enzyme inhibition activities and chemical profiles of Polygonum sachalinensis F.Schmidt ex Maxim (Polygonaceae) |
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| Authors: | Peihong Fan Lara Terrier Anne-Emmanuelle Hay Andrew Marston Kurt Hostettmann |
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| Institution: | 1. Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland;2. Department of Natural Product Chemistry, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China |
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| Abstract: | Polygonum sachalinensis is a widespread invasive plant in Europe. Chemical profiles of its different organs were studied by HPLC-UV-ESI/MS. Seven major constituents quercetin-3-O-β-D-galactopyranoside, quercetin-3-O-arabinopyranoside, lapathoside D, N-trans-feruloyltyramine, lapathoside C, hydropiperoside, and vanicoside B were isolated and identified. The free radical-scavenging, α/β-glucosidase, and acetylcholinesterase inhibitory activities of crude MeOH extracts and isolated compounds were studied. The structure–activity relationships were discussed. The chemical profiles revealed flavonoids and phenylpropanoids are the major compounds of all the organs of this plant. Quercetin-3-O-arabinopyranoside, lapathoside D, N-trans-feruloyltyramine, lapathoside C and hydropiperoside were isolated from this species for the first time. In the α-glucosidase bioassay, quercetin-3-O-β-D-galactopyranoside, lapathoside D and N-trans-feruloyltyramine demonstrated stronger activities than the positive reference acarbose. The trend in scavenging power showed no relation to enzyme inhibition in the test models. |
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| Keywords: | α-PNPG p-nitrophenyl-α-D-glucopyranoside β-PNPG p-nitrophenyl-β-D-glucopyranoside AChE Acetylcholinesterase ATCI Acetyl thiocholine iodide BSA Bovine serum albumin CID Collision-induced dissociation DAD Photodiode array detector DPPH 1 1-diphenyl-2-picrylhydrazyl DTNB Dithio-bis-(2-nitrobenzoic acid) ELISA Enzyme-linked immunosorbent assay ESI Electrospray ionization HPLC High performance liquid chromatography IC50 The concentration of substance that provides 50% inhibition to certain reaction MPLC Medium pressure liquid chromatography MS Mass spectrometry NMR Nuclear magnetic resonance TOF Time of flight UV Ultra Violet |
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