| 一株高效溶解钾长石芽孢杆菌的分离鉴定与生物学特性研究 |
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| 引用本文： | 谢庆东,何琳燕,王 琪,盛下放.一株高效溶解钾长石芽孢杆菌的分离鉴定与生物学特性研究[J].土壤,2017,49(2):302-307. |
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| 作者姓名： | 谢庆东 何琳燕 王 琪 盛下放 |
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| 作者单位： | 农业部农业环境微生物实验室,南京农业大学生命科学学院,南京 210095 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘 要： | 从风化的钾质粗面岩表面分离筛选到1株高效风化钾长石的芽孢杆菌E31菌株,通过菌株的生理生化特征并结合菌株16S r DNA序列分析,E31菌株被鉴定为苏云金芽孢杆菌(Bacillus thuringiensis)。同时对E31菌株溶解钾长石的效应与机制以及生物学特性进行了研究。结果表明,E31菌株能风化钾长石并释放出其中的元素,28℃振荡培养不同时间,接菌处理发酵液中可溶性K、Fe、Ca、Al含量分别比对照提高了19.6%~25.6%、1~12.5倍、10.4%~47.2%和1.2~4.5倍。在钾长石存在条件下接菌处理发酵液pH为3.62~3.80,发酵液中葡萄糖酸含量达61~1 794 mg/L,发酵液细胞数量达(8.7~16.1)×10~6 cfu/ml,表明菌株可以通过代谢产生的有机酸来加速对钾长石的分解作用。另外,E31菌株能够合成生长素和铁载体,E31菌株对温度、pH和盐浓度具有较强的耐受性。
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| 关 键 词： | 钾质粗面岩 钾长石 苏云金芽孢杆菌 矿物风化 葡萄糖酸 生物学特性 |
| 收稿时间： | 2016/4/20 0:00:00 |
| 修稿时间： | 2016/5/4 0:00:00 |
Isolation and Identification of a Feldspar-dissolving Bacillus Strain and its Biological Characteristics |
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| XIE Qingdong,HE Lianyan,WANG Qi and SHENG Xiafang.Isolation and Identification of a Feldspar-dissolving Bacillus Strain and its Biological Characteristics[J].Soils,2017,49(2):302-307. |
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| Authors: | XIE Qingdong HE Lianyan WANG Qi and SHENG Xiafang |
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| Institution: | Key Laboratory of Agricultural and Environmental Microbiology,Ministry of Agriculture,College of Life Science,Nanjing Agricultural University,Key Laboratory of Agricultural and Environmental Microbiology,Ministry of Agriculture,College of Life Science,Nanjing Agricultural University,Key Laboratory of Agricultural and Environmental Microbiology,Ministry of Agriculture,College of Life Science,Nanjing Agricultural University,Key Laboratory of Agricultural and Environmental Microbiology,Ministry of Agriculture,College of Life Science,Nanjing Agricultural University |
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| Abstract: | One mineral-weathering Bacillus strain was isolated from the surface of weathered potash trachyte and was identified as Bacillus thuringiensis based on the physiological and biochemical traits and 16S rDNA sequence analysis. The dissolution of feldspar by strain E31 and the mechanism involved as well as the biological characteristics were characterized. The results showed that strain E31 could weather feldspar and release elements from the mineral. The concentrations of K, Fe, Ca, and Al were increased by 19.6-25.6%, 1-12.5-fold, 10.4-47.2% and 1.2-4.5-fold in the presence of strain E31 compared with the controls respectively during the mineral weathering process. The pH value, gluconic acid content, and cell numbers in the culture medium were 3.62-3.80, 61-1794 mg/L, and 8.7-16.1×106 cfu/mL, respectively, in the presence of strain E31. Furthermore, E31 strain had the ability to produce IAA and siderophores in culture medium. E31 strain had also the characteristics of acid or alkali and salt tolerance and temperature resistance. |
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| Keywords: | Potash trachyte Feldspar Bacillus thuringiensis Mineral weathering Gluconic acid Biological characteristics |
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