| 临床分离的动物源链球菌对红霉素的蓄积动力学研究 |
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| 引用本文： | 王丽平,陆承平.临床分离的动物源链球菌对红霉素的蓄积动力学研究[J].畜牧兽医学报,2007,38(2):202-205. |
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| 作者姓名： | 王丽平 陆承平 |
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| 作者单位： | 南京农业大学动物医学院,南京,210095 |
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| 基金项目： | 国家“973”项目子专题（G1999011906） |
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| 摘 要： | 为证实国内临床分离的动物源链球菌菌株中是否存在与红霉素耐药性相关的主动外排机制，选取含有mefA基因、ermB基因、不含mefA／ermB基因的链球菌耐药菌株和青霉素、红霉素均敏感的菌株，采用完整细胞物质转运动力学方法研究了菌株对红霉素的主动外排功能。结果表明，含mefA基因的菌株对红霉素的主动外排作用显著增强，对红霉素的蓄积浓度显著低于其它菌株（P〈0．05），且氰氯苯腙（CCCP）可减少红霉素的主动外排作用，增加菌体内红霉素的蓄积浓度（P〈0．05）。本试验结果佐证了在链球菌内存在与红霉素耐药相关的主动外排机制。
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| 关 键 词： | 动物源链球菌 红霉素 主动外排 完整细胞物质转运动力学 红霉素蓄积 |
| 文章编号： | 0366-6964（2007）02-0202-04 |
| 修稿时间： | 2006-05-15 |
Accumulation Dynamics of Erythromycin in Streptococcus Strains Isolated from Veterinary Clinic |
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| WANG Li-ping,LU Cheng-ping.Accumulation Dynamics of Erythromycin in Streptococcus Strains Isolated from Veterinary Clinic[J].Acta Veterinaria et Zootechnica Sinica,2007,38(2):202-205. |
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| Authors: | WANG Li-ping LU Cheng-ping |
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| Institution: | College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | To identify if there was the mechanism of active efflux in erythromycin resistant Streptococcus strains with mefA gene isolated from veterinary clinic,several erythromycin resistant strains with mefA gene,ermB gene and several susceptible strains were selected in this test.The uptake of 14C-erythromycin in all strains and the effects of carbonylcyanidem chlorophenylhydrazone(CCCP,a proton conductor) on the erythromycin accumulation were assayed with intact cells.The results showed that increased active exocytosis and decreased accumulation of erythromycin(P<0.05) were occurred in strains with mefA gene,and CCCP led reverse results.The accumulation and the response to the addition of a proton conductor indicated that the erythromycin resistant strains with mefA gene could actively pump out 14C-erythromycin from the cell. |
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| Keywords: | Streptococcus suis erythromycin active efflux dynamics of substance transport in intact cell erythromycin accumulation |
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