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湖北省香菇自然种群交配型因子分析
引用本文:汪中文,林芳灿.湖北省香菇自然种群交配型因子分析[J].食用菌学报,1996,3(4):4-10.
作者姓名:汪中文  林芳灿
作者单位:华中农业大学!武汉430070
摘    要:以在湖北省无栽培菌株孢子传播的香菇自然发生地随机采集的32个野生香菇菌株为材料,用原生质体单核体和孢子单核体配对相结合的方法分析鉴定样本中的交配型因子,并用统计学方法分析了菌丝原生质体单核体。

关 键 词:香菇  交配型因子  种群  种质资源

Analysis of the Mating Type Factors in Natural Population of Lentinula edodes in Hubei Province
Wang Zhongwen,Lin Fangcan.Analysis of the Mating Type Factors in Natural Population of Lentinula edodes in Hubei Province[J].Acta Edulis Fungi,1996,3(4):4-10.
Authors:Wang Zhongwen  Lin Fangcan
Abstract:Mating type factors in 32 strains of Lentinula edodes collected from mountainous region of Hubei Province without dissemination of spores of cultivated strains were determined by the mating test between spore monokaryons and protoplast monokaryons derived from binuclear mycelia. In addition, analyses of the mating type ratio of protoplast monokaryons and spore monokaryons and the distribution frequency of specific mating factors in the samples were made with statistical methods. The results indicated that both the expected 1:1 ratio of mating types of protoplast monokaryons and the expected 1:1:1:1 ratio of mating types of spore monokaryons were obtained in most of the strains. 52 distinct A factors and 55 distinct B factors were determined from the sample and the series of both the specific A factors and B factors were randomly distributed. The replication frequency of mating factor in wild strains was much lower than that in cultivated strains. It meant that there was abundant germplasm resource in the natural population of Lentinula edodes. Therefore, it was important that the wild strains be served as the primary breeding materials for improving genetic variability of commercial strains of Lentinula edodes.
Keywords:Lentinula edodes  Mating type factor  Natural population  Germplasm resource
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