| 几种营养条件对裂褶菌生长及产L—苹果酸的影响 |
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| 引用本文： | 钱秀萍,杨庆尧.几种营养条件对裂褶菌生长及产L—苹果酸的影响[J].食用菌学报,1996,3(3):18-24. |
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| 作者姓名： | 钱秀萍 杨庆尧 |
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| 作者单位： | 上海师范大学生物系，上海师范大学生物系 上海 200234，上海 200234 |
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| 摘 要： | 裂褶菌在发酵培养基中振荡培养产生苹果酸的最佳碳源是葡萄糖,每毫升发酵液产苹果酸10.48mg,果糖和麦芽糖次之,苹果酸产量分别为6.28mg/ml、5.05mg/m1.最佳氮源是牛肉浸膏、草酸铵和硝酸铵,苹果酸产量分别为6.65mg/ml。6.05mg/ml、5.98mg/ml,苹果酸产率分别是16.5%、13.3%、12.5%.在自来水配制的发酵培养基中,磷酸二氢钾和氯化钾对裂褶菌产酸影响不大,而培养基中添加硫酸镁后苹果酸产量下降。碳酸钙浓度与苹果酸产量成正比,碳酸钙浓度从1%增加到5%,苹果酸产量从1.55mg/ml提高到11.38mg/ml,产率可提高约7倍。
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| 关 键 词： | 裂褶菌 发酵 苹果酸 碳源 氮源 无机盐 |
Effects of Some Nutrient Conditions on Mycelium Growth and L-malic Acid Production of Schizophyllum commune |
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| Qian Xiuping Yang Qingyao.Effects of Some Nutrient Conditions on Mycelium Growth and L-malic Acid Production of Schizophyllum commune[J].Acta Edulis Fungi,1996,3(3):18-24. |
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| Authors: | Qian Xiuping Yang Qingyao |
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| Abstract: | L-malic acid could be produced by Schizophyllum commune through shake-culture in fermentative medium. It was proved that the best carbon source was glucose producing 10.48mg/ml malic acid in the fermentative medium, followed by fructose and maltose with a malic acid yield of 6.28mg/ml and 5.05 mg/ml resp.. The most favourable nitrogen sources were proved to be beef extract,ammonium oxalate and ammonium nitrate. Their malic acid yields were .6.65mg/ml, 6.05mg/ml and 5.98mg/ml resp. with the productive rate being 16.5%, 13.3% and 12.5% resp.. It was found that KH2PO4 and KC1 hardly affected Schizophyllum commune to produce acid in fermentative medium made up with tap water , but MgSO4 decreased the yield of malic acid. When the concentration of CaCO3 was raised from 1% to 5%, the malic acid yield was increased from 1.55mg/ml to 11.38mg/ml and the productive rate was increased by a factor of about 7. |
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| Keywords: | Schizophyllum commune Fermentation Malic acid Carbon source Nitrogen source Inorganic salt |
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