| 脱氧雪腐镰刀菌烯醇(DON)抗原化分子设计及其检测 |
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| 引用本文： | 王希春,邢云云,金福源,刘海明,何成华,樊彦红,张海彬.脱氧雪腐镰刀菌烯醇(DON)抗原化分子设计及其检测[J].畜牧与兽医,2009,41(10). |
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| 作者姓名： | 王希春 邢云云 金福源 刘海明 何成华 樊彦红 张海彬 |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏,南京,210095;安徽农业大学动物科技学院,安徽,合肥,230036
2. 南京农业大学动物医学院,江苏,南京,210095 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用琥珀酸酐法对DON分子进行结构修饰,采用高效液相色谱法和薄层层析法进行检测,并将衍生物与载体蛋白牛血清白蛋白进行偶联,设计人工抗原,通过紫外分光光度法、SDS-PAGE和ELISA试剂盒分析偶联结果。结果显示,成功得到了DON-BSA,偶联物中DON浓度为0.797μg/μL。
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇 人工抗原 鉴定 |
Deoxynivalenol (DON) antigenic molecular design and its identification |
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| WANG Xi-chun,XING Yun-yun,JIN Fu-yuan,LIU Hai-ming,HE Cheng-hua,FAN Yan-hong,ZHANG Hai-bin.Deoxynivalenol (DON) antigenic molecular design and its identification[J].Animal Husbandry & Veterinary Medicine,2009,41(10). |
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| Authors: | WANG Xi-chun XING Yun-yun JIN Fu-yuan LIU Hai-ming HE Cheng-hua FAN Yan-hong ZHANG Hai-bin |
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| Abstract: | Deoxynivalenol(DON) molecular structure was modified with succinic anhydride(SA) method in this study.HPLC and TLC were used to determine the product.The derivative and carrier protein BSA were coupled to develop artificial antigen.The conjugate was analyzed with ultraviolet spectrophotometry,SDS-PAGE and ELISA kit for DON.The result showed that DON artificial antigen was obtained and the content of DON in DON-BSA was 0.797 μg/μL. |
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| Keywords: | deoxynivalenol(DON) artificial antigen identification |
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