| CpG DNA对鸡传染性喉气管炎病毒DNA疫苗免疫效果的影响 |
| |
| 引用本文： | 孟松树,李曦,智海东,王云峰,王柳,童光志.CpG DNA对鸡传染性喉气管炎病毒DNA疫苗免疫效果的影响[J].中国农业科学,2001,34(3):342-344. |
| |
| 作者姓名： | 孟松树 李曦 智海东 王云峰 王柳 童光志 |
| |
| 作者单位： | 中国农业科学院哈尔滨兽医研究所 |
| |
| 基金项目： | 中华农业科教人才基金及中国农业科学院跨世纪人才基金 |
| |
| 摘 要： | 将分别构建的含有鸡传染性喉气管炎病毒（ＩＬＴＶ）王岗株ｇＢｇＣ和ｇＤ基因的重组真核表达质粒及ＣＰＧＤＮＡ佐剂分组肌肉注射 ＳＰＦ鸡，检测了免疫后的抗体水平，并观测了攻毒后的免疫保护效果。实验结果显示，ＣｐＧＤＮＡ佐剂和ＤＮＡ疫苗联合免疫后的抗体水平比单一使用ＤＮＡ疫苗的要高，而佐剂组的发病率、死亡率均低于非佐剂组，保护率则高于非佐剂组。这表明 ＣｐＧ ＤＮＡ佐剂增强了 ＩＬＴＶ ＤＮＡ疫苗的免疫效果。
|
| 关 键 词： | 传染性喉气管炎病毒 DNA疫苗 CpG DNA 免疫效果 |
| 文章编号： | 0578-1752(2001)03-0342-03 |
| 修稿时间： | 1999年12月28 |
Contribution of CpG DNA to the Immune Effect Induced by DNA Vaccines Against Infectious Laryngotracheitis Virus |
| |
| MENG Song-shu,LI Xi,ZHI Hai-dong,WANG Yun-feng,WANG Liu,TONG Guang-zhi.Contribution of CpG DNA to the Immune Effect Induced by DNA Vaccines Against Infectious Laryngotracheitis Virus[J].Scientia Agricultura Sinica,2001,34(3):342-344. |
| |
| Authors: | MENG Song-shu LI Xi ZHI Hai-dong WANG Yun-feng WANG Liu TONG Guang-zhi |
| |
| Abstract: | Chickens of different groups were immunized by intramuscular injection of eukaryotic expression recombinant plasmids containing gB. gC and gD genes of infectious laryngotracheitis virus (ILTV) Wanggang strain (DNA vaccines), the above DNA vaccines used in two groups of chickens were mixed with CpG DNA extracted from E. coli . as adjuvants. Specific antibody was detected after immunization and immune effects were observed after challenge. Results showed that the antibody titer detected in chickens immunized with DNA vaccine and CpG DNA were higher than those of chickens without use of CpG DNA, and both morbidity and mortality of chickens with use of CpG DNA were lower than those of chickens without use of CpG DNA. The above results indicated that the immune effect of DNA vaccine against ILTV was enhanced by CpG DNA as adjuvants. |
| |
| Keywords: | Infectious laryngotracheitis virus DNA vaccine CpG DNA Immune effect |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
