| 新疆野生油菜BAC文库的构建 |
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| 引用本文： | 薄存沛,闫晓红,徐有明,胡琼,伍晓明,魏文辉.新疆野生油菜BAC文库的构建[J].中国油料作物学报,2010,32(1):14. |
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| 作者姓名： | 薄存沛 闫晓红 徐有明 胡琼 伍晓明 魏文辉 |
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| 作者单位： | 1.华中农业大学园艺林学学院，湖北 武汉，430070；
2.中国农业科学院油料作物研究所基因组学与分子生物学研究室，农业部油料作物生物学重点开放实验室，湖北 武汉，430062
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| 基金项目： | 国家自然科学基金，湖北省自然科学基金 |
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| 摘 要： | 本实验以新疆野生油菜18号的基因组DNA为材料构建了其BAC(bacterial artificial chromosome)文库。该文库共有14592个克隆,插入片段在50-300kb之间,平均插入片段105kb,空载率低于5%,覆盖了新疆野生油菜基因组约4.0倍。新疆野生油菜基因组BAC文库的构建,为进一步研究其基因组结构及功能基因的利用等奠定了基础。
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| 关 键 词： | 新疆野生油菜 BAC文库 基因组 功能基因 |
Construction of the bacterial artificial chromosome library of Xinjiang wild rape(Sinapis arvensis) |
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| BO Cun-pei,YAN Xiao-hong,XU You-ming,HU Qiong,WU Xiao-ming,WEI Wen-hui.Construction of the bacterial artificial chromosome library of Xinjiang wild rape(Sinapis arvensis)[J].Chinese Journal of Oil Crop Sciences,2010,32(1):14. |
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| Authors: | BO Cun-pei YAN Xiao-hong XU You-ming HU Qiong WU Xiao-ming WEI Wen-hui |
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| Institution: | BO Cun-pei1,2,YAN Xiao-hong2,XU You-ming1,HU Qiong2,WU Xiao-ming2,WEI Wen-hui2(1.College of Horticulture , Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China,2.Department of Genomics , Molecular Biology of Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Oil Crop Biology of the Ministry of Agriculture,Wuhan 430062,China) |
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| Abstract: | Xinjiang wild rape (Sinapis arvensis) is an important germplasm resource for rapeseed breeding.It distributes in Xinjiang Uyghur Autonomous Region of China.A genomic bacterial artificial chromosome (BAC) library was constructed using nuclear DNA of Xinjiang Yeyou 18.The library collected 14 592 recombinant clones carrying inserts with sizes ranging from 50kb to 300kb. The average insert size was about 105kb with empty-vector rate less than 5.0%. This BAC library represented 4 equivalents of Xinjiang wild ra... |
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| Keywords: | Xinjiang wild rape BAC library Genome Functional gene |
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