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| 红富士苹果叶片不定芽再生中激素、多胺和NO含量的变化 | |
| 引用本文： | 田春英,邵建柱,刘莹,徐继忠.红富士苹果叶片不定芽再生中激素、多胺和NO含量的变化[J].园艺学报,2010,37(9):1403-1408. |
| 作者姓名： | 田春英 邵建柱 刘莹 徐继忠 |
| 作者单位： | 1. 河北农业大学园艺学院,河北保定,071000;承德市农业广播电视学校,河北承德,067000 2. 河北农业大学园艺学院,河北保定,071000 3. 河北农业大学园艺学院,河北保定,071000;承德石油高等专科学校,河北承德,067000 |
| 摘 要： | 以红富士苹果继代试管苗叶片为试材,对光、暗培养条件下叶片不定芽再生过程中内源激素、内源多胺及NO含量变化进行了测定。研究结果表明:暗培养下内源ZR和ABA及内源多胺的水平显著高于光培养。叶片接种初期诱导细胞启动分化时ZR、IAA和ABA含量较高,细胞旺盛分裂期及芽原基形成期ZR和ABA含量较高而IAA含量较低。与光培养相比,暗培养下ZR/IAA和ABA/IAA均较高。在叶片接种初期(0～7d)内源腐胺(Put)、精胺(Spm)、亚精胺(Spd)以及内源多胺总量均达到峰值,并且在不定芽分化时期内源多胺含量始终维持在较高水平。与光培养相比,暗培养叶片的NO含量较高。红富士苹果叶片不定芽再生与其内源激素、多胺和NO含量密切相关。 |
| 关 键 词： | 苹果 叶片 不定芽再生 内源激素 内源多胺 一氧化氮 |
Changes of Hormones,Polyamines and NO Content During Regeneration of Adventitious Buds from in Vitro Leaves of Red Fuji Apple |
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| TIAN Chun-ying,SHAO Jian-zhu,LIU Ying,XU Ji-zhong.Changes of Hormones,Polyamines and NO Content During Regeneration of Adventitious Buds from in Vitro Leaves of Red Fuji Apple[J].Acta Horticulturae Sinica,2010,37(9):1403-1408. | |
| Authors: | TIAN Chun-ying SHAO Jian-zhu LIU Ying XU Ji-zhong |
| Institution: | （1College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071001，China；2Agricultural Broadcasting and Television College of Hebei，Chengde，Hebei 067000，China；3Chengde Petroleum College of Hebei，Chengde，Hebei 067000，China） |
| Abstract: | This study was conducted with leaves collected from the test-tube plantlet of Red Fuji apple as the experimental materials. Changes of hormones，polyamines and NO during in vitro regeneration of adventitious buds were studied under light and dark culture. The results indicated that the contents of ZR，ABA and polyamines under dark culture were higher than that under light culture. The hormone（ZR，ABA and IAA）contents in leaves had the high level during the early stage of inoculation，and the higher level of ZR and ABA，the lower level of IAA were found at the stage of cell division and bud primodium formation. Meanwhile，the ratios of ZR/IAA and ABA/IAA under dark culture were both higher than that under light culture. During the initial stage of inoculation，the endogenous polyamines contents had reached peak，and the contents was also in higher level at the stage of adventitious buds differentiation. During the culture of leaves in vitro，the contents of NO under dark culture kept higher than that of light culture. It is obvious that adventitious bud formation from in vitro leaves of Red Fuji apple was closely associated with contents of endogenous hormones，polyamines and NO. |
| Keywords: | apple leaves regeneration of adventitious bud endogenous hormone endogenous polyamine nitric oxide |
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