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| 不同冷冻方法对牛卵泡卵母细胞发育潜力的影响 | |
| 引用本文： | 于永生,常万存,李鹏,严娟,郝少强,李青旺.不同冷冻方法对牛卵泡卵母细胞发育潜力的影响[J].中国牛业科学,2002,28(2):21-23. |
| 作者姓名： | 于永生 常万存 李鹏 严娟 郝少强 李青旺 |
| 作者单位： | 1. 山东省农科院高新技术中心,山东济南,250100西北农林科技大学,畜牧兽医学院,陕西杨凌,712100 2. 山东省农科院高新技术中心,山东济南,250100 3. 山东中大胚胎中心,山东曹县,274400 4. 西北农林科技大学,畜牧兽医学院,陕西杨凌,712100 |
| 基金项目： | 山东省自然科学基金(Q99D06) |
| 摘 要： | 本试验探讨了不同方法冷冻保存的牛卵泡卵母细胞体外受精后的发育潜力。尽管用不同方法冷冻的牛卵母细胞大多（79．1％）形态正常，但体外受精后的受精率（13．2％）和卵裂率（8．8％）均明显低于对照组（66．7％和46．0％），卵裂后继续发育的能力严重受损。在3种方法中，玻璃化冷冻效果最好，受精率分别达到15．0％和11．1％，是值得进一步研究的一种方法。 |
| 关 键 词： | 牛 卵泡卵母细胞 冷冻方法 发育潜力 保种 |
| 文章编号： | 1001-9111(2002)02-0021-03 |
| 修稿时间： | 2001年10月30 |
Effects of Different Cryopreservation Methods on the Development Competences of Bovine Follicular Oocytes |
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| YU Yong sheng\\{,\},CHANG Wan cun\,LI Peng\,YAN Juan\,HAO Shao qiang\,LI Qing wang\.Effects of Different Cryopreservation Methods on the Development Competences of Bovine Follicular Oocytes[J].China Cattle Science,2002,28(2):21-23. | |
| Authors: | YU Yong sheng\\{ \} CHANG Wan cun\ LI Peng\ YAN Juan\ HAO Shao qiang\ LI Qing wang\ |
| Institution: | YU Yong sheng\+\{1,3\},CHANG Wan cun\+1,LI Peng\+2,YAN Juan\+2,HAO Shao qiang\+2,LI Qing wang\+3 |
| Abstract: | The developmental competences of bovine follicular oocytes cryopreserved by different methods were studied.Although most of the oocytes(79.1%) appeared morphologically normal after freezing and thawing,the fertilization rate(13.2%)and cleavage rate(8.8%) were significantly lower than those of control group(66.7% and 46.0%).after in vitro insemination and the developmental competences of 2-cell embryos derived from cryopreserved oocytes was also severely damaged.among the 3 cryopreservation methods, vitrification method achieved the highest fertilization rate(15.0%) and cleavage rate(11.1%),and this method was worthy of further study. |
| Keywords: | Bovine Follicular oocytes Cryopreservation method Developmental competence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |