Analysis of clonal germplasm from five Saccharum species: S. barberi , S. robustum, S. officinarum, S. sinense and S. spontaneum . A study of inter- and intra species relationships using microsatellite markers |
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| Authors: | J Steven Brown R J Schnell E J Power Stephanie L Douglas and David N Kuhn |
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| Institution: | (1) Subtropical Horticulture Research Station, U. S. Department of Agriculture, Agricultural Research Service, 13601 Old Cutler Road, Miami, FL 33158, USA;(2) Department of Biological Sciences, Florida International University, Miami, FL 33199, USA |
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| Abstract: | In order to study relationships within and among species of the highly complex polyploid genus Saccharum, 30 clones from 5 species (S. barberi, S. officinarum, S. robustum, S. sinense, and S. spontaneum) and 6 cultivated clones were analyzed using 15 sugarcane simple sequence repeat (SSR) markers and two multivariate statistical
methods. The origins of two species, S. barberi and S. sinense, are unclear, and it has been hypothesized that they arose from crosses of S. officinarum × S. spontaneum. S. robustum is suggested to be ancestral to S. officinarum, which, when crossed with S. spontaneum, principally comprise the genomes of commercial cultivars. Although our analyses supported S. robustum as being an ancestor of S. officinarum, and show the composition of commercial clones as expected, our results concerning the species S. barberi and S. sinense indicated them to be much less related to the main Saccharum germplasm pool than previous molecular marker investigations, suggesting that introgression from other genera may exist in
their ancestry. The differing results of our analysis of S. barberi and S. sinense are likely attributable to more equal and larger numbers of clones studied in each species. Given the extremely high ploidy,
the use of only 15 SSR markers enabled clear distinction of Saccharum species and clones, and also identified likely mislabeled accessions. In addition, the replication of DNA extraction, polymerase
chain reactions, and fragment electrophoresis increased the robustness of our analysis. |
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| Keywords: | Saccharum barberi Saccharum sinense Saccharum ancestors Saccharum species interrelationships Sugarcane SSR profiles |
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