| 小麦合成种M53抗白粉病基因的RAPD和SSR标记 |
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| 引用本文： | 胡英考,辛志勇.小麦合成种M53抗白粉病基因的RAPD和SSR标记[J].作物学报,2001,27(4):415-419. |
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| 作者姓名： | 胡英考 辛志勇 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所，农业部作物遗传育种重点开放实验室，北京 100081 |
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| 基金项目： | 本研究由国家自然科学基金(编号：39893350)和973项目(编号：G1998010205)资助 |
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| 摘 要： | 运用RAPD和SSR技术，采用分离群体分组分析法(BSA)进行了小麦合成种M53抗白粉病基因连锁的分子标记研究。结果表明，M53的抗白粉病基因由显性单基因控制，RAPD标记OPL09-1700与抗病基因连锁，遗传距离为16.8cM。SSR标记Xgwm205也与抗白粉病基因连锁，遗传距离为9.3cM，通过SSR标记将该基因定位于5DS，标记与基因间的排列顺序
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| 关 键 词： | 合成种小麦 抗白粉病 基因 RAPD SSR |
| 收稿时间： | 2000-07-28 |
| 修稿时间： | 2000年7月28日 |
RAPD and SSR Markers Linked to Powdery Mildew Resistance Gene in Triticum
durum-Aegilops squarrosa Amphidiploid M53 |
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| HU Ying,Kao,XIN Zhi,YongWTBX.RAPD and SSR Markers Linked to Powdery Mildew Resistance Gene in Triticum
durum-Aegilops squarrosa Amphidiploid M53[J].Acta Agronomica Sinica,2001,27(4):415-419. |
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| Authors: | HU Ying Kao XIN Zhi Yong[WTBX] |
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| Institution: | HU Ying Kao XIN Zhi Yong[WT6BX] |
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| Abstract: | Random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) were employed to detect molecular markers linked to powdery mildew resistance gene in Triticum durum Aegilops squarrosa amphidiploid M53. The resistance was controlled by single dominant gene. A RAPD marker OPL09 -1700 was proved to link to the resistance gene with genetic distance of 16.8 cM. A SSR marker Xgwm205 was also proved to link to the resistance gene and the genetic distance is 9.3 cM. This powdery mildew resistance gene has been located in 5DS and the sequence in 5DS is OPL09 -1700 Pm Xgwm205. |
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| Keywords: | Synthetic wheat Powdery mildew Gene RAPD SSR |
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