| 高效液相色谱法测定猪肝组织中3-甲基喹喔啉-2-羧酸 |
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| 引用本文： | 姚蕴珊,沈建忠,吴聪明,程林丽.高效液相色谱法测定猪肝组织中3-甲基喹喔啉-2-羧酸[J].中国畜牧兽医,2011,38(2):187-190. |
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| 作者姓名： | 姚蕴珊 沈建忠 吴聪明 程林丽 |
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| 作者单位： | (中国农业大学动物医学院药理与毒理研究组，北京 100193) |
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| 基金项目： | 公益性行业(农业)科研专项经费“动物卫生风险分析关键技术与应用研究” |
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| 摘 要： | 本研究建立了猪肝组织中3-甲基喹喔啉-2羧酸（3-methyl-quinoxaline-2-carboxylic acid,MQCA）的高效液相色谱检测方法。采用0.3 mol/L盐酸提取猪肝组织中药物，经MAX柱净化，以乙腈∶甲醇∶0.5%甲酸水（15∶50∶35，V/V/V）为流动相，C18色谱柱进行药物分离，320 nm波长下紫外检测，结果当药物在猪肝组织中添加水平为20～250 μg/kg时，平均回收率为72.35%～85.00%，日内测定的相对标准偏差（RSD）（n＝5）为5.56%～9.49%，日间RSD(n＝3)为5.96%～9.63%。猪肝组织中MQCA的定量限为20 μg/kg，检出限为6.65 μg/kg，表明该方法灵敏度、准确度和精密度均符合兽药残留分析技术的要求，适用于猪肝组织中MQCA的检测。
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| 关 键 词： | 高效液相色谱法 猪肝组织 3-甲基喹喔啉-2-羧酸 |
Determination of 3-methyl-quinoxaline-2-carboxylic Acid in Porcine Liver by High Performance Liquid Chromatography |
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| YAO Yun-shan,SHEN Jian-zhong,WU Cong-ming,CHENG Lin-li.Determination of 3-methyl-quinoxaline-2-carboxylic Acid in Porcine Liver by High Performance Liquid Chromatography[J].China Animal Husbandry & Veterinary Medicine,2011,38(2):187-190. |
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| Authors: | YAO Yun-shan SHEN Jian-zhong WU Cong-ming CHENG Lin-li |
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| Institution: | (Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China) |
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| Abstract: | To develop a high performance liquid chromatography method for determination of 3-methyl-quinoxaline-2-carboxylic acid(MQCA) in porcine liver.MQCA was extracted from porcine liver with 0.3 mol/L hydrochloric acid.The extract was cleaned up on MAX Sep-Pak Silica solid-phase extraction cartridge.Then the eluate was collected and dried under a gentle stream of nitrogen gas and the dissolved residue was analyzed by HPLC.The HPLC separation was performed on a Symmetry Shield reversed phase C18 boned silica colum... |
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| Keywords: | high performance liquid chromatography(HPLC) porcine liver MQCA |
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