| 叶斑病对黄精叶片内生真菌群落结构的影响 |
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| 引用本文： | 陈美琪,王越,尹慧君,赵文琪,梁绪康,王信行,胡正雨,李子安,郑家雪,李艳玲,田园.叶斑病对黄精叶片内生真菌群落结构的影响[J].现代农业科技,2023(15). |
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| 作者姓名： | 陈美琪 王越 尹慧君 赵文琪 梁绪康 王信行 胡正雨 李子安 郑家雪 李艳玲 田园 |
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| 作者单位： | 山东第一医科大学,,,,,,,,,, |
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| 基金项目： | 山东省医药卫生科技发展计划(202101060623),山东省大学生创新创业计划项目(基于高通量测序的黄精叶斑病微观机制解析及其拮抗菌的生防作用探究),山东第一医科大学(山东省医学科学院)青年科学基金培育资助计划(202201-034) |
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| 摘 要： | 为了探究健康和叶斑病害黄精叶片内生真菌群落的结构差异，采用Illumina NovaSeq技术对健康（JK）和染病（RB）黄精叶片内真菌的ITS rDNA进行测序，分析两组样品物种多样性和群落结构。结果显示，RB组叶片内物种多样性较JK组无显著变化，但物种丰度较JK组显著降低。RB组中的优势菌群为担孢酵母属、刺盘孢属、链格孢属和宙斯沸耳属，而JK组中的附球霉属、毛壳菌属和枝孢属的比例显著高于RB组。本研究证明了叶斑病害会导致染病黄精叶片内生真菌群落的丰度下降，使某些有害物种优势生长，该研究为黄精叶斑病病害的防治提供了理论依据。
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| 关 键 词： | 黄精 叶斑病 内生真菌 群落结构 |
| 收稿时间： | 2022/11/29 0:00:00 |
| 修稿时间： | 2022/11/29 0:00:00 |
Effects of Polygonatum sibiricum Leaf Spot Disease on Endophytic Fungal Community Structure |
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| Authors: | Tian Yuan |
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| Institution: | College of Life Science,Shandong First Medical University Shandong Academy of Medical Sciences,Taian |
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| Abstract: | This study aims to explore the difference of endophyte community structure between healthy and leaf spot effected Polygonatum sibiricum. Illumina NovaSeq technology was used to sequence the ITS rDNA of fungi in healthy (JK) and infected (RB) P. sibiricum leaves, and the species diversity and community structure of the two groups of samples were analyzed. The species diversity in RB group was not significantly different from that in JK group, but the species abundance was significantly lower than that in JK group. The dominant genus in RB group were Erythrobasidium, Colletotrichum, Alternaria, and Dioszegia, while the proportions of Epicoccum, Chaetomium, and Cladosporium in JK group were significantly higher than those in RB group. The abundance of endophytic fungi community in infected leaves decreased due to leaf spot disease, which led to the growth of some harmful species. This study provided a theoretical basis for the prevention and control of leaf spot disease of P. sibiricum. |
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| Keywords: | Polygonatum sibiricum leaf spot disease endophytic fungi community structure |
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